Efficient Derivation of Embryonic Stem Cells from Nuclear Transfer and Parthenogenetic Embryos Derived from Cryopreserved Oocytes

Li-Ying Sung, Ching-Chien Chang, Tomokazu Amano, Chih-Jen Lin, Misa Amano, Stephen B. Treaster, Jie Xu, Wei-Fang Chang, Zsolt Peter Nagy, Xiangzhong Yang, X. Cindy Tian*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Deriving histocompatible embryonic stem (ES) cells by somatic cell nuclear transfer (SCNT) and parthenogenetic activation (PA) requires fresh oocytes, which prevents their applications in humans. Here, we evaluated the efficiency of deriving ES cells from mature metaphase II (MII) and immature metaphase I (MI) vitrified oocytes, by PA or SCNT, in a mouse model. We successfully generated ES cell lines from PA (MII and MI) and SCNT (MII and MI) blastocysts. These cell lines expressed genes and antigens characteristic of pluripotent ES cells and produced full-term pups upon tetraploid embryo complementation. This study established an animal model for efficient generation of patient-specific ES cell lines using cryopreserved oocytes. This is a major step forward in the application of therapeutic cloning and parthenogenetic technology in human regenerative medicine and will serve as an important alternative to the iPS cell technology in countries/regions where these technologies are permitted.

Original languageEnglish
Pages (from-to)203-211
Number of pages9
JournalCellular Reprogramming
Volume12
Issue number2
DOIs
Publication statusPublished - Apr 2010

Keywords

  • IN-VITRO
  • THERAPEUTIC CLONING
  • SOMATIC-CELLS
  • MOUSE OOCYTES
  • PARKINSONIAN MICE
  • ES CELLS
  • 1,2-PROPANEDIOL
  • VITRIFICATION
  • PREGNANCY
  • DISEASE

Cite this