Efficient Production of Human beta-Defensin 2 (HBD2) in Escherichia coli

Thomas Vargues; Gareth J. Morrison; Emily S. Seo; David J. Clarke; Helen L. Fielder; Julien Bennani; Uday Pathania; Fiona Kilanowski; Julia R. Dorin; John R. W. Govan; C. Logan Mackay; Dusan Uhrin; Dominic J. Campopiano

doi:10.2174/092986609788490122

Efficient Production of Human beta-Defensin 2 (HBD2) in Escherichia coli

Thomas Vargues, Gareth J. Morrison, Emily S. Seo, David J. Clarke, Helen L. Fielder, Julien Bennani, Uday Pathania, Fiona Kilanowski, Julia R. Dorin, John R. W. Govan, C. Logan Mackay, Dusan Uhrin, Dominic J. Campopiano

Research output: Contribution to journal › Article › peer-review

Abstract

Human beta-defensin 2 (HBD2) has been shown to interact with pathogenic bacteria and components of the mammalian innate and adaptive immune response. We describe a quick and reliable method for the production of HBD2 in Escherichia coli. HBD2 was expressed as an insoluble fusion, chemically cleaved and oxidised to give a single, folded HBD2 beta-isoform. The purified peptide was analysed by high resolution mass spectrometry, displayed a well-dispersed H-1 NMR spectrum, was a chemoattractant to HEK293 cells expressing CCR6 and acted as an antimicrobial agent against E. coli, P. aeruginosa, C. albicans and S. aureus.

Original language	English
Pages (from-to)	668-676
Number of pages	9
Journal	Protein and Peptide Letters
Volume	16
Issue number	6
DOIs	https://doi.org/10.2174/092986609788490122
Publication status	Published - Jun 2009

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title = "Efficient Production of Human beta-Defensin 2 (HBD2) in Escherichia coli",

abstract = "Human beta-defensin 2 (HBD2) has been shown to interact with pathogenic bacteria and components of the mammalian innate and adaptive immune response. We describe a quick and reliable method for the production of HBD2 in Escherichia coli. HBD2 was expressed as an insoluble fusion, chemically cleaved and oxidised to give a single, folded HBD2 beta-isoform. The purified peptide was analysed by high resolution mass spectrometry, displayed a well-dispersed H-1 NMR spectrum, was a chemoattractant to HEK293 cells expressing CCR6 and acted as an antimicrobial agent against E. coli, P. aeruginosa, C. albicans and S. aureus.",

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T1 - Efficient Production of Human beta-Defensin 2 (HBD2) in Escherichia coli

AU - Vargues, Thomas

AU - Morrison, Gareth J.

AU - Seo, Emily S.

AU - Clarke, David J.

AU - Fielder, Helen L.

AU - Bennani, Julien

AU - Pathania, Uday

AU - Kilanowski, Fiona

AU - Dorin, Julia R.

AU - Govan, John R. W.

AU - Mackay, C. Logan

AU - Uhrin, Dusan

AU - Campopiano, Dominic J.

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AB - Human beta-defensin 2 (HBD2) has been shown to interact with pathogenic bacteria and components of the mammalian innate and adaptive immune response. We describe a quick and reliable method for the production of HBD2 in Escherichia coli. HBD2 was expressed as an insoluble fusion, chemically cleaved and oxidised to give a single, folded HBD2 beta-isoform. The purified peptide was analysed by high resolution mass spectrometry, displayed a well-dispersed H-1 NMR spectrum, was a chemoattractant to HEK293 cells expressing CCR6 and acted as an antimicrobial agent against E. coli, P. aeruginosa, C. albicans and S. aureus.

U2 - 10.2174/092986609788490122

DO - 10.2174/092986609788490122

M3 - Article

SN - 0929-8665

VL - 16

SP - 668

EP - 676

JO - Protein and Peptide Letters

JF - Protein and Peptide Letters

IS - 6

ER -

Efficient Production of Human beta-Defensin 2 (HBD2) in Escherichia coli

Abstract

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