Efficient Production of Human beta-Defensin 2 (HBD2) in Escherichia coli

Thomas Vargues, Gareth J. Morrison, Emily S. Seo, David J. Clarke, Helen L. Fielder, Julien Bennani, Uday Pathania, Fiona Kilanowski, Julia R. Dorin, John R. W. Govan, C. Logan Mackay, Dusan Uhrin, Dominic J. Campopiano

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Human beta-defensin 2 (HBD2) has been shown to interact with pathogenic bacteria and components of the mammalian innate and adaptive immune response. We describe a quick and reliable method for the production of HBD2 in Escherichia coli. HBD2 was expressed as an insoluble fusion, chemically cleaved and oxidised to give a single, folded HBD2 beta-isoform. The purified peptide was analysed by high resolution mass spectrometry, displayed a well-dispersed H-1 NMR spectrum, was a chemoattractant to HEK293 cells expressing CCR6 and acted as an antimicrobial agent against E. coli, P. aeruginosa, C. albicans and S. aureus.

Original languageEnglish
Pages (from-to)668-676
Number of pages9
JournalProtein and Peptide Letters
Volume16
Issue number6
DOIs
Publication statusPublished - Jun 2009

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