Projects per year
Abstract / Description of output
The green alga Chlamydomonas reinhardtii is an invaluable reference organism to research fields including algal, plant, and ciliary biology. Accordingly, decades-long standing inefficiencies in targeted nuclear gene editing broadly hinder Chlamydomonas research. Here we report that single-step codelivery of CRISPR/Cpf1 ribonucleoproteins with single-stranded DNA repair templates results in precise and targeted DNA replacement with as much as ∼10% efficiency in C. reinhardtii. We demonstrate its use in transgene- and selection-free generation of sequence-specific mutations and epitope tagging at an endogenous locus. As the direct delivery of gene-editing reagents bypasses the use of transgenes, this method is potentially applicable to a wider range of species without the need to develop methods for stable transformation.
Original language | English |
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Pages (from-to) | 13567-13572 |
Number of pages | 6 |
Journal | Proceedings of the National Academy of Sciences (PNAS) |
Volume | 114 |
Issue number | 51 |
Early online date | 5 Dec 2017 |
DOIs | |
Publication status | Published - 19 Dec 2017 |
Keywords / Materials (for Non-textual outputs)
- Chlamydomonas reinhardtii
- CRISPR/Cpf1
- RNP
- editing
- ssODN
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Dive into the research topics of 'Efficient targeted DNA editing and replacement in Chlamydomonas reinhardtii using Cpf1 ribonucleoproteins and single-stranded DNA'. Together they form a unique fingerprint.Projects
- 1 Finished
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High value chemicals from algae: Developing a transgene-free genome editing toolbox to enhance carotenoid production
1/08/16 → 30/09/17
Project: Research
Profiles
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Attila Molnar
- School of Biological Sciences - Senior Lecturer
- Centre for Engineering Biology
Person: Academic: Research Active