Electrochemical reduction of flavocytochromes 2B4 and 1A2 and their catalytic activity

V V Shumyantseva, T V Bulko, T T Bachmann, U Bilitewski, R D Schmid, A I Archakov, Till Bachmann

Research output: Contribution to journalArticlepeer-review

Abstract

The present study shows that cytochromes P450 2B4 and 1A2 with a covalently attached riboflavin (semisynthetic flavocytochromes RfP450 2B4 and RfP450 1A2) can be reduced electrochemically on rhodium-graphite electrodes at a potential of -500 mV (vs Ag/AgCl). In the presence of substrates such as aminopyrine, aniline, 7-ethoxyresorufin, and 7-pentoxyresorufin, N-demethylation, p-hydroxylation, and O-dealkylation reactions proceeded, as was confirmed by product analysis. Rates of electrocatalytically driven reactions are comparable to those obtained using NAD(P)H as the source of reducing equivalents. These results suggest the practicality of developing flavocytochrome P450s as catalysts for oxidation reactions with different classes of organic substrates.
Original languageEnglish
Pages (from-to)43-8
Number of pages6
JournalArchives of biochemistry and biophysics
Volume377
Issue number1
DOIs
Publication statusPublished - 2000

Keywords

  • Alkylation
  • Aminopyrine
  • Aniline Compounds
  • Animals
  • Aryl Hydrocarbon Hydroxylases
  • Catalysis
  • Cytochrome P-450 CYP1A2
  • Cytochrome P-450 Enzyme System
  • Electrodes
  • Electrolysis
  • Graphite
  • Hydroxylation
  • Kinetics
  • Male
  • Methylation
  • NAD
  • NADP
  • Oxazines
  • Oxidation-Reduction
  • Rabbits
  • Reducing Agents
  • Rhodium
  • Steroid Hydroxylases

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