Electrochemical sensing of human neutrophil elastase and polymorphonuclear neutrophil activity

Eva González-Fernández, Matteo Staderini, Amirah Yussof, Emma Scholefield, Alan F. Murray, Andrew R. Mount*, Mark Bradley

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Human neutrophil elastase (HNE) is a serine protease, produced by polymorphonuclear neutrophils (PMNs), whose uncontrolled production has been associated with various inflammatory disease states as well as tumour proliferation and metastasis. Here we report the development and characterisation of an electrochemical peptide-based biosensor, which enables the detection of clinically relevant levels of HNE. The sensing platform was characterised in terms of its analytical performance, enzymatic cleavage kinetics and cross-reactivity and applied to the quantitative detection of protease activity from PMNs from human blood.

Original languageEnglish
Pages (from-to)209-214
Number of pages6
JournalBiosensors and Bioelectronics
Volume119
Early online date9 Aug 2018
DOIs
Publication statusPublished - 15 Nov 2018

Keywords / Materials (for Non-textual outputs)

  • Electrochemical detection
  • Human neutrophil elastase
  • Methylene blue-tagged peptides
  • Protease detection
  • Self-assembled monolayer

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