Eliminating caspase-7 and cathepsin B cross-reactivity on fluorogenic caspase-3 substrates

Martha Mackay, Ana Perez-Lopez, Mark Bradley, Annamaria Lilienkampf

Research output: Contribution to journalArticlepeer-review

Abstract

11 FRET-based fluorogenic substrates were constructed using the pentapeptide template Asp-Glu-X2-Asp-X10, and evaluated with caspase-3, caspase-7 and cathepsin B. The sequence Asp-Glu-Pro-Asp-Ser was able to selectively quantify caspase-3 activity in vitro without notable caspase-7 and cathepsin B cross-reactivity, while exhibiting low lM KM values and good catalytic efficiencies (7.0–16.9 lM 1 min 1).
Original languageEnglish
Pages (from-to)693-696
JournalMolecular BioSystems
Volume12
Issue number3
DOIs
Publication statusPublished - 12 Dec 2015

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