emc has a role in dorsal appendage fate formation in Drosophila oogenesis

Sofia Papadia, George Tzolovsky, Debiao Zhao, Kevin Leaper, Dorothy Clyde, Paul Taylor, Eva Asscher, Graeme Kirk, Mary Bownes

Research output: Contribution to journalArticlepeer-review

Abstract

extramacrochaetae (emc) functions during many developmental processes in Drosophila, such as sensory organ formation, sex determination, wing vein differentiation, regulation of eye photoreceptor differentiation, cell proliferation and development of the Malpighian tubules, trachea and muscles in the embryo. It encodes a Helix-Loop-Helix transcription factor that negatively regulates bHLH proteins. We show here that emc mRNA and protein are present throughout oogenesis in a dynamic expression pattern and that emc is involved in the regulation of chorionic appendage formation during late oogenesis. Expression of sense and antisense emc constructs as well as emc follicle cell clones leads to eggs with shorter, thicker dorsal appendages that are closer together at base than in the wild type. We demonstrate that emc lies downstream of fs(1)K10, gurken and EGFR in the Grk/EGFR signalling pathway and that it participates in controlling Broad-Complex expression at late stages of oogenesis.
Original languageEnglish
Pages (from-to)961-74
Number of pages14
JournalMechanisms of Development
Volume122
Issue number9
Publication statusPublished - Sep 2005

Keywords

  • Animals
  • Animals, Genetically Modified
  • Basic Helix-Loop-Helix Transcription Factors
  • Chorion/growth & development
  • DNA, Antisense/genetics
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/metabolism
  • Drosophila/genetics
  • Drosophila/growth & development
  • Drosophila/metabolism
  • Drosophila Proteins/genetics
  • Drosophila Proteins/metabolism
  • Female
  • Gene Expression Regulation, Developmental
  • Genes, Insect
  • Mutation
  • Oogenesis/genetics
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Signal Transduction

Fingerprint Dive into the research topics of 'emc has a role in dorsal appendage fate formation in Drosophila oogenesis'. Together they form a unique fingerprint.

Cite this