Emergence of human angiohematopoietic cells in normal development and from cultured embryonic stem cells

Elias T. Zambidis, Lidia Sinka, Manuela Tavian, Venta Jokubaitis, Tea Soon Park, Paul Simmons, Bruno Peault

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Human hematopoiesis proceeds transiently in the extraembryonic yolk sac and embryonic, then fetal liver before being stabilized in the bone marrow during the third month of gestation. In addition to this classic developmental sequence, we have previously shown that the aorta-gonad-mesonephros (AGM) embryonic territory produces stem cells for definitive hematopoiesis from 27 to 40 days of human development, through an intermediate blood-forming endothelium stage. These studies have relied on the use of traditional markers of human hematopoietic and endothelial cells. In addition, we have recently identified and characterized a novel surface molecule, 131139, which typifies the earliest founders of the human angiohematopoietic system. 13139, which was initially identified with a monoclonal antibody raised to Stro-1(+) bone marrow stromal cells, recognizes in the adult the most primitive Thy-1(+) CD133(+) Lin(-), non-obese diabetic-severe combined immunodeficiency disease (NOD-SCID) mouse engrating hematopoietic stem cells (HSCs). In the 3- to 4-week embryo, 13139 expression typifies a subset of splanchnopleural mesodermal cells that migrate dorsally and colonize the ventral aspect of the aorta where they establish a population of hemogenic endothelial cells. We have indeed confirmed that hematopoietic potential in the human embryo, as assessed by long-term culture-initiating cell (LTC-IC) and SCID mouse reconstituting cell (SRC) activities, is confined to BB9-expressing cells. We have further validated these results in the model of human embryonic stem cells (hESCs) in which we have modeled, through the development of hematopoietic embryoid bodies (EBs), primitive and definitive hematopoieses. In this setting, we have documented the emergence of BB9(+) hemangioblast-like clonogenic angiohematopoietic progenitors that currently represent the earliest known founders of the human vascular and blood systems.

Original languageEnglish
Title of host publicationHematopoietic Stem Cells VI
Subtitle of host publicationThe Annals of the New York Academy of Sciences
EditorsL Kanz, KC Weisel, JE Dick, WE Fibbe
Place of PublicationOXFORD
Number of pages10
ISBN (Print)978-1-57331-676-7
Publication statusPublished - 2007
Event6th Biennial International Symposium and Workshop on Hematopoietic Stem Cells - Tubingen
Duration: 14 Sep 200616 Sep 2006


Conference6th Biennial International Symposium and Workshop on Hematopoietic Stem Cells


  • hematopoietic stem cell
  • Embryonic stem cell
  • hemangioblast
  • Blood vessel


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