Endothelial-specific Gata3 expression is required for hematopoietic stem cell generation

Nada Zaidan; Leslie Nitsche; Evangelia Diamanti; Rebecca Hannah; Antonella Fidanza; Nicola K Wilson; Lesley M Forrester; Berthold Gottgens; Katrin Ottersbach

doi:10.1016/j.stemcr.2022.06.008

Endothelial-specific Gata3 expression is required for hematopoietic stem cell generation

Nada Zaidan, Leslie Nitsche, Evangelia Diamanti, Rebecca Hannah, Antonella Fidanza, Nicola K Wilson, Lesley M Forrester, Berthold Gottgens, Katrin Ottersbach^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Original language	English
Journal	Stem Cell Reports
DOIs	https://doi.org/10.1016/j.stemcr.2022.06.008
Publication status	Published - 12 Jul 2022

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Cite this

@article{9b27fc0985f24457ab7d97d6cd2483ba,

title = "Endothelial-specific Gata3 expression is required for hematopoietic stem cell generation",

author = "Nada Zaidan and Leslie Nitsche and Evangelia Diamanti and Rebecca Hannah and Antonella Fidanza and Wilson, {Nicola K} and Forrester, {Lesley M} and Berthold Gottgens and Katrin Ottersbach",

note = "Funding Information: The authors are very grateful to the staff of the animal facilities both at the Cambridge Institute for Medical Research and the Center for Regenerative Medicine for their support with animal experiments and to the flow cytometry teams at both of these institutes, Dr. Reiner Schulte, Dr. Chiara Cossetti, and Michal Maj in Cambridge and Fiona Rossi and Dr Claire Cryer in Edinburgh, as well as the Cambridge NIHR BRC Cell Phenotyping Hub, for excellent cell sorting services and help with flow cytometry analyses. We would further like to acknowledge the assistance of Matthew Gratian and Mark Bowen (Cambridge Institute for Medical Research) and Bertrand Vernay (Center for Regenerative Medicine) with microscopy, and Drs C{\'e}line Souilhol and Stanislav Rybtsov with setting up the co-aggregate assays. We are also indebted to Jingfang Zhu and Meinrad Busslinger for providing the conditional Gata3 KO and the Gata3-GFP mice, respectively. Core facilities at the Edinburgh Centre for Regenerative Medicine were supported by center grant MR/K017047/1 . This work was funded by an Intermediate Fellowship from the Kay Kendall Leukaemia Fund (to K.O.), a Blood Cancer UK Bennett Senior Fellowship ( 10015 to K.O.), a fellowship from the King Abdullah International Medical Research Center ( KAIMRC ), Ministry of National Guard Health Affairs (to N.Z.) and a Wellcome Trust studentship (to L.N.). This research was also funded in part by the Wellcome Trust and the UKRI Medical Research Council . For the purpose of open access, the author has applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission. Funding Information: The authors are very grateful to the staff of the animal facilities both at the Cambridge Institute for Medical Research and the Center for Regenerative Medicine for their support with animal experiments and to the flow cytometry teams at both of these institutes, Dr. Reiner Schulte, Dr. Chiara Cossetti, and Michal Maj in Cambridge and Fiona Rossi and Dr Claire Cryer in Edinburgh, as well as the Cambridge NIHR BRC Cell Phenotyping Hub, for excellent cell sorting services and help with flow cytometry analyses. We would further like to acknowledge the assistance of Matthew Gratian and Mark Bowen (Cambridge Institute for Medical Research) and Bertrand Vernay (Center for Regenerative Medicine) with microscopy, and Drs C{\'e}line Souilhol and Stanislav Rybtsov with setting up the co-aggregate assays. We are also indebted to Jingfang Zhu and Meinrad Busslinger for providing the conditional Gata3 KO and the Gata3-GFP mice, respectively. Core facilities at the Edinburgh Centre for Regenerative Medicine were supported by center grant MR/K017047/1. This work was funded by an Intermediate Fellowship from the Kay Kendall Leukaemia Fund (to K.O.), a Blood Cancer UK Bennett Senior Fellowship (10015 to K.O.), a fellowship from the King Abdullah International Medical Research Center (KAIMRC), Ministry of National Guard Health Affairs (to N.Z.) and a Wellcome Trust studentship (to L.N.). This research was also funded in part by the Wellcome Trust and the UKRI Medical Research Council. For the purpose of open access, the author has applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

month = jul,

day = "12",

doi = "10.1016/j.stemcr.2022.06.008",

language = "English",

journal = "Stem Cell Reports",

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T1 - Endothelial-specific Gata3 expression is required for hematopoietic stem cell generation

AU - Zaidan, Nada

AU - Nitsche, Leslie

AU - Diamanti, Evangelia

AU - Hannah, Rebecca

AU - Fidanza, Antonella

AU - Wilson, Nicola K

AU - Forrester, Lesley M

AU - Gottgens, Berthold

AU - Ottersbach, Katrin

N1 - Funding Information: The authors are very grateful to the staff of the animal facilities both at the Cambridge Institute for Medical Research and the Center for Regenerative Medicine for their support with animal experiments and to the flow cytometry teams at both of these institutes, Dr. Reiner Schulte, Dr. Chiara Cossetti, and Michal Maj in Cambridge and Fiona Rossi and Dr Claire Cryer in Edinburgh, as well as the Cambridge NIHR BRC Cell Phenotyping Hub, for excellent cell sorting services and help with flow cytometry analyses. We would further like to acknowledge the assistance of Matthew Gratian and Mark Bowen (Cambridge Institute for Medical Research) and Bertrand Vernay (Center for Regenerative Medicine) with microscopy, and Drs Céline Souilhol and Stanislav Rybtsov with setting up the co-aggregate assays. We are also indebted to Jingfang Zhu and Meinrad Busslinger for providing the conditional Gata3 KO and the Gata3-GFP mice, respectively. Core facilities at the Edinburgh Centre for Regenerative Medicine were supported by center grant MR/K017047/1 . This work was funded by an Intermediate Fellowship from the Kay Kendall Leukaemia Fund (to K.O.), a Blood Cancer UK Bennett Senior Fellowship ( 10015 to K.O.), a fellowship from the King Abdullah International Medical Research Center ( KAIMRC ), Ministry of National Guard Health Affairs (to N.Z.) and a Wellcome Trust studentship (to L.N.). This research was also funded in part by the Wellcome Trust and the UKRI Medical Research Council . For the purpose of open access, the author has applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission. Funding Information: The authors are very grateful to the staff of the animal facilities both at the Cambridge Institute for Medical Research and the Center for Regenerative Medicine for their support with animal experiments and to the flow cytometry teams at both of these institutes, Dr. Reiner Schulte, Dr. Chiara Cossetti, and Michal Maj in Cambridge and Fiona Rossi and Dr Claire Cryer in Edinburgh, as well as the Cambridge NIHR BRC Cell Phenotyping Hub, for excellent cell sorting services and help with flow cytometry analyses. We would further like to acknowledge the assistance of Matthew Gratian and Mark Bowen (Cambridge Institute for Medical Research) and Bertrand Vernay (Center for Regenerative Medicine) with microscopy, and Drs Céline Souilhol and Stanislav Rybtsov with setting up the co-aggregate assays. We are also indebted to Jingfang Zhu and Meinrad Busslinger for providing the conditional Gata3 KO and the Gata3-GFP mice, respectively. Core facilities at the Edinburgh Centre for Regenerative Medicine were supported by center grant MR/K017047/1. This work was funded by an Intermediate Fellowship from the Kay Kendall Leukaemia Fund (to K.O.), a Blood Cancer UK Bennett Senior Fellowship (10015 to K.O.), a fellowship from the King Abdullah International Medical Research Center (KAIMRC), Ministry of National Guard Health Affairs (to N.Z.) and a Wellcome Trust studentship (to L.N.). This research was also funded in part by the Wellcome Trust and the UKRI Medical Research Council. For the purpose of open access, the author has applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission. The authors declare no competing interests. Publisher Copyright: © 2022 The Author(s)

PY - 2022/7/12

Y1 - 2022/7/12

U2 - 10.1016/j.stemcr.2022.06.008

DO - 10.1016/j.stemcr.2022.06.008

M3 - Article

SN - 2213-6711

JO - Stem Cell Reports

JF - Stem Cell Reports

ER -