Endothelium-derived hyperpolarizing factor and potassium use different mechanisms to induce relaxation of human subcutaneous resistance arteries

C A McIntyre; C H Buckley; G C Jones; T C Sandeep; R C Andrews; A I Elliott; G A Gray; B C Williams; J A McKnight; B R Walker; P W Hadoke

doi:10.1038/sj.bjp.0704143

Endothelium-derived hyperpolarizing factor and potassium use different mechanisms to induce relaxation of human subcutaneous resistance arteries

C A McIntyre, C H Buckley, G C Jones, T C Sandeep, R C Andrews, A I Elliott, G A Gray, B C Williams, J A McKnight, B R Walker, P W Hadoke

Research output: Contribution to journal › Article › peer-review

Abstract

This investigation examined the hypothesis that release of K(+) accounts for EDHF activity by comparing relaxant responses produced by ACh and KCl in human subcutaneous resistance arteries. Resistance arteries (internal diameter 244+/-12 microm, n=48) from human subcutaneous fat biopsies were suspended in a wire myograph. Cumulative concentration-response curves were obtained for ACh (10(-9) - 3x10(-5) M) and KCl (2.5 - 25 mM) following contraction with noradrenaline (NA; 0.1 - 3 microM). ACh (E(max) 99.07+/-9.61%; -LogIC(50) 7.03+/-0.22; n=9) and KCl (E(max) 74.14+/-5.61%; -LogIC(50) 2.12+/-0.07; n=10)-induced relaxations were attenuated (P

Original language	English
Pages (from-to)	902-8
Number of pages	7
Journal	British Journal of Pharmacology
Volume	133
Issue number	6
DOIs	https://doi.org/10.1038/sj.bjp.0704143
Publication status	Published - 2001

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McIntyre, C. A., Buckley, C. H., Jones, G. C., Sandeep, T. C., Andrews, R. C., Elliott, A. I., Gray, G. A., Williams, B. C., McKnight, J. A., Walker, B. R., & Hadoke, P. W. (2001). Endothelium-derived hyperpolarizing factor and potassium use different mechanisms to induce relaxation of human subcutaneous resistance arteries. British Journal of Pharmacology, 133(6), 902-8. https://doi.org/10.1038/sj.bjp.0704143

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title = "Endothelium-derived hyperpolarizing factor and potassium use different mechanisms to induce relaxation of human subcutaneous resistance arteries",

abstract = "This investigation examined the hypothesis that release of K(+) accounts for EDHF activity by comparing relaxant responses produced by ACh and KCl in human subcutaneous resistance arteries. Resistance arteries (internal diameter 244+/-12 microm, n=48) from human subcutaneous fat biopsies were suspended in a wire myograph. Cumulative concentration-response curves were obtained for ACh (10(-9) - 3x10(-5) M) and KCl (2.5 - 25 mM) following contraction with noradrenaline (NA; 0.1 - 3 microM). ACh (E(max) 99.07+/-9.61%; -LogIC(50) 7.03+/-0.22; n=9) and KCl (E(max) 74.14+/-5.61%; -LogIC(50) 2.12+/-0.07; n=10)-induced relaxations were attenuated (P",

author = "McIntyre, {C A} and Buckley, {C H} and Jones, {G C} and Sandeep, {T C} and Andrews, {R C} and Elliott, {A I} and Gray, {G A} and Williams, {B C} and McKnight, {J A} and Walker, {B R} and Hadoke, {P W}",

year = "2001",

doi = "10.1038/sj.bjp.0704143",

language = "English",

volume = "133",

pages = "902--8",

journal = "British Journal of Pharmacology",

issn = "0007-1188",

publisher = "Nature Publishing Group",

number = "6",

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McIntyre, CA, Buckley, CH, Jones, GC, Sandeep, TC, Andrews, RC, Elliott, AI, Gray, GA, Williams, BC, McKnight, JA, Walker, BR & Hadoke, PW 2001, 'Endothelium-derived hyperpolarizing factor and potassium use different mechanisms to induce relaxation of human subcutaneous resistance arteries', British Journal of Pharmacology, vol. 133, no. 6, pp. 902-8. https://doi.org/10.1038/sj.bjp.0704143

TY - JOUR

T1 - Endothelium-derived hyperpolarizing factor and potassium use different mechanisms to induce relaxation of human subcutaneous resistance arteries

AU - McIntyre, C A

AU - Buckley, C H

AU - Jones, G C

AU - Sandeep, T C

AU - Andrews, R C

AU - Elliott, A I

AU - Gray, G A

AU - Williams, B C

AU - McKnight, J A

AU - Walker, B R

AU - Hadoke, P W

PY - 2001

Y1 - 2001

N2 - This investigation examined the hypothesis that release of K(+) accounts for EDHF activity by comparing relaxant responses produced by ACh and KCl in human subcutaneous resistance arteries. Resistance arteries (internal diameter 244+/-12 microm, n=48) from human subcutaneous fat biopsies were suspended in a wire myograph. Cumulative concentration-response curves were obtained for ACh (10(-9) - 3x10(-5) M) and KCl (2.5 - 25 mM) following contraction with noradrenaline (NA; 0.1 - 3 microM). ACh (E(max) 99.07+/-9.61%; -LogIC(50) 7.03+/-0.22; n=9) and KCl (E(max) 74.14+/-5.61%; -LogIC(50) 2.12+/-0.07; n=10)-induced relaxations were attenuated (P

AB - This investigation examined the hypothesis that release of K(+) accounts for EDHF activity by comparing relaxant responses produced by ACh and KCl in human subcutaneous resistance arteries. Resistance arteries (internal diameter 244+/-12 microm, n=48) from human subcutaneous fat biopsies were suspended in a wire myograph. Cumulative concentration-response curves were obtained for ACh (10(-9) - 3x10(-5) M) and KCl (2.5 - 25 mM) following contraction with noradrenaline (NA; 0.1 - 3 microM). ACh (E(max) 99.07+/-9.61%; -LogIC(50) 7.03+/-0.22; n=9) and KCl (E(max) 74.14+/-5.61%; -LogIC(50) 2.12+/-0.07; n=10)-induced relaxations were attenuated (P

U2 - 10.1038/sj.bjp.0704143

DO - 10.1038/sj.bjp.0704143

M3 - Article

C2 - 11454664

VL - 133

SP - 902

EP - 908

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 0007-1188

IS - 6

ER -

Endothelium-derived hyperpolarizing factor and potassium use different mechanisms to induce relaxation of human subcutaneous resistance arteries

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