Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore

Tomoyuki Ohba; Eric C Schirmer; Takeharu Nishimoto; Larry Gerace

doi:10.1083/jcb.200409149

Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore

Tomoyuki Ohba, Eric C Schirmer, Takeharu Nishimoto, Larry Gerace

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Resident integral proteins of the inner nuclear membrane (INM) are synthesized as membrane-integrated proteins on the peripheral endoplasmic reticulum (ER) and are transported to the INM throughout interphase using an unknown trafficking mechanism. To study this transport, we developed a live cell assay that measures the movement of transmembrane reporters from the ER to the INM by rapamycin-mediated trapping at the nuclear lamina. Reporter constructs with small (

Original language	English
Pages (from-to)	1051-62
Number of pages	12
Journal	Journal of Cell Biology
Volume	167
Issue number	6
DOIs	https://doi.org/10.1083/jcb.200409149
Publication status	Published - 2004

Keywords / Materials (for Non-textual outputs)

Adenosine Triphosphate
Endoplasmic Reticulum
Energy Metabolism
HeLa Cells
Humans
Membrane Proteins
Nuclear Lamina
Nuclear Pore
Nuclear Pore Complex Proteins
Nuclear Proteins
Protein Transport
Sirolimus
Temperature
Time Factors

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10.1083/jcb.200409149

Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore
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title = "Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore",

abstract = "Resident integral proteins of the inner nuclear membrane (INM) are synthesized as membrane-integrated proteins on the peripheral endoplasmic reticulum (ER) and are transported to the INM throughout interphase using an unknown trafficking mechanism. To study this transport, we developed a live cell assay that measures the movement of transmembrane reporters from the ER to the INM by rapamycin-mediated trapping at the nuclear lamina. Reporter constructs with small (",

keywords = "Adenosine Triphosphate, Endoplasmic Reticulum, Energy Metabolism, HeLa Cells, Humans, Membrane Proteins, Nuclear Lamina, Nuclear Pore, Nuclear Pore Complex Proteins, Nuclear Proteins, Protein Transport, Sirolimus, Temperature, Time Factors",

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T1 - Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore

AU - Ohba, Tomoyuki

AU - Schirmer, Eric C

AU - Nishimoto, Takeharu

AU - Gerace, Larry

PY - 2004

Y1 - 2004

N2 - Resident integral proteins of the inner nuclear membrane (INM) are synthesized as membrane-integrated proteins on the peripheral endoplasmic reticulum (ER) and are transported to the INM throughout interphase using an unknown trafficking mechanism. To study this transport, we developed a live cell assay that measures the movement of transmembrane reporters from the ER to the INM by rapamycin-mediated trapping at the nuclear lamina. Reporter constructs with small (

AB - Resident integral proteins of the inner nuclear membrane (INM) are synthesized as membrane-integrated proteins on the peripheral endoplasmic reticulum (ER) and are transported to the INM throughout interphase using an unknown trafficking mechanism. To study this transport, we developed a live cell assay that measures the movement of transmembrane reporters from the ER to the INM by rapamycin-mediated trapping at the nuclear lamina. Reporter constructs with small (

KW - Adenosine Triphosphate

KW - Endoplasmic Reticulum

KW - Energy Metabolism

KW - HeLa Cells

KW - Humans

KW - Membrane Proteins

KW - Nuclear Lamina

KW - Nuclear Pore

KW - Nuclear Pore Complex Proteins

KW - Nuclear Proteins

KW - Protein Transport

KW - Sirolimus

KW - Temperature

KW - Time Factors

U2 - 10.1083/jcb.200409149

DO - 10.1083/jcb.200409149

M3 - Article

C2 - 15611332

SN - 0021-9525

VL - 167

SP - 1051

EP - 1062

JO - Journal of Cell Biology

JF - Journal of Cell Biology

IS - 6

ER -

Energy- and temperature-dependent transport of integral proteins to the inner nuclear membrane via the nuclear pore

Abstract

Keywords / Materials (for Non-textual outputs)

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