Engineering and improvement of the efficiency of a chimeric [P450cam-RhFRed reductase domain] enzyme

Aélig Robin, Gareth A Roberts, Johannes Kisch, Federico Sabbadin, Gideon Grogan, Neil Bruce, Nicholas J Turner, Sabine L Flitsch

Research output: Contribution to journalArticlepeer-review

Abstract

A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.

Original languageEnglish
Pages (from-to)2478-2480
Number of pages3
JournalChemical Communications
Issue number18
Early online date24 Mar 2009
DOIs
Publication statusPublished - 14 May 2009

Keywords

  • Camphor 5-Monooxygenase/chemistry
  • Oxidoreductases/chemistry
  • Protein Engineering/methods
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins/chemistry
  • Rhodococcus/enzymology
  • Transformation, Genetic

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