A chimeric oxygenase, in which the P450cam domain was fused to the reductase host domains of a P450RhF from Rhodococcus sp. strain NCIMB 9784 was optimised to allow for a biotransformation at 30 mM substrate in 80% overall yield, with the linker region between P450 and FMN domain proving to be important for the effective biotransformation of (+)-camphor to 5-exo-hydroxycamphor.
- Camphor 5-Monooxygenase/chemistry
- Protein Engineering/methods
- Protein Structure, Tertiary
- Recombinant Fusion Proteins/chemistry
- Transformation, Genetic