Enhanced Angiogenic Capacity of Human Umbilical Vein Endothelial Cells From Women With Preeclampsia

Amie J. Moyes, David Maldonado-Perez, Gillian A. Gray, Fiona C. Denison

Research output: Contribution to journalArticlepeer-review

Abstract

Maternal and placental angiogenic abnormalities are a common feature of preeclampsia. The aim of this study was to determine if endothelial cells from women with preeclampsia exhibit different angiogenic responses compared to healthy cells. Using the endothelial tube formation assay, we have shown that primary human umbilical vein endothelial cells (HUVECs) isolated from women with preeclampsia display greater levels of in vitro angiogenic branching compared to cells from healthy women. A comparable increase in tube formation was observed in healthy cells cultured at 0.5% O-2. Vascular endothelial growth factor (VEGF) receptor inhibition resulted in a decrease in angiogenesis in both healthy hypoxic cells and cells from women with preeclampsia. These findings demonstrate that HUVECs from women with preeclampsia exhibit inherent differences in their angiogenic capacity which are apparent in the absence of placental or maternal factors.

Original languageEnglish
Pages (from-to)374-382
Number of pages9
JournalReproductive Sciences
Volume18
Issue number4
DOIs
Publication statusPublished - Apr 2011

Keywords

  • angiogenesis
  • preeclampsia
  • endothelium
  • VEGF
  • hypoxia

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