Enhanced secretion of interferon-gamma by bovine gammadelta T cells induced by coculture with Mycobacterium bovis-infected dendritic cells: evidence for reciprocal activating signals

S.J. Price, J.C. Hope

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Evidence suggests that gammadelta T cells form part of the innate immune response to Mycobacterium bovis infection. Dendritic cells (DCs) are capable of secreting high levels of interleukin-12 (IL-12) following infection with mycobacteria and can induce interferon-gamma (IFN-gamma) secretion by natural killer and gammadelta T cells We investigated the innate interactions occurring between WC1(+)gammadelta T cells and M. bovis-infected DCs. Following coculture with M. bovis-infected DCs, secretion of IFN-gamma and expression of CD25 and major histocompatibility complex class II on WC1(+)gammadelta T cells were significantly enhanced. Reciprocal enhancement of IL-12 secretion by the DCs was also observed and this interaction was found to be contact dependent. We hypothesize that there is an early, transient signal between the WC1(+)gammadelta T cells and the DCs, which promotes the synthesis of biologically active IL-12, and which is dependent upon cell-cell contact. Reciprocal signals including IL-12 are then delivered to WC1(+)gammadelta cells, which leads to the enhanced secretion of IFN-gamma, and the up-regulation of activation markers and antigen presentation molecules by the WC1(+)gammadelta T cells. These interactions are likely to form a critical part of the T helper type 1-conditioning response of DCs to M. bovis.
Original languageUndefined/Unknown
Pages (from-to)201-208
Number of pages8
JournalImmunology
Volume126
Issue number2
DOIs
Publication statusPublished - 2009

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Cattle
  • Cell Communication/immunology
  • Cells, Cultured
  • Coculture Techniques
  • Dendritic Cells/immunology
  • Dendritic Cells/microbiology
  • Histocompatibility Antigens Class II/metabolism
  • Interferon-gamma/metabolism
  • Interleukin-12/metabolism
  • Interleukin-2 Receptor alpha Subunit/metabolism
  • Lymphocyte Activation/immunology
  • Mycobacterium bovis/immunology
  • Receptors, Antigen, T-Cell, gamma-delta/analysis
  • T-Lymphocyte Subsets/immunology
  • Tuberculosis, Bovine/immunology
  • Up-Regulation/immunology

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