Abstract / Description of output
Evidence suggests that gammadelta T cells form part of the innate immune response to Mycobacterium bovis infection. Dendritic cells (DCs) are capable of secreting high levels of interleukin-12 (IL-12) following infection with mycobacteria and can induce interferon-gamma (IFN-gamma) secretion by natural killer and gammadelta T cells We investigated the innate interactions occurring between WC1(+)gammadelta T cells and M. bovis-infected DCs. Following coculture with M. bovis-infected DCs, secretion of IFN-gamma and expression of CD25 and major histocompatibility complex class II on WC1(+)gammadelta T cells were significantly enhanced. Reciprocal enhancement of IL-12 secretion by the DCs was also observed and this interaction was found to be contact dependent. We hypothesize that there is an early, transient signal between the WC1(+)gammadelta T cells and the DCs, which promotes the synthesis of biologically active IL-12, and which is dependent upon cell-cell contact. Reciprocal signals including IL-12 are then delivered to WC1(+)gammadelta cells, which leads to the enhanced secretion of IFN-gamma, and the up-regulation of activation markers and antigen presentation molecules by the WC1(+)gammadelta T cells. These interactions are likely to form a critical part of the T helper type 1-conditioning response of DCs to M. bovis.
Original language | Undefined/Unknown |
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Pages (from-to) | 201-208 |
Number of pages | 8 |
Journal | Immunology |
Volume | 126 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2009 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Cattle
- Cell Communication/immunology
- Cells, Cultured
- Coculture Techniques
- Dendritic Cells/immunology
- Dendritic Cells/microbiology
- Histocompatibility Antigens Class II/metabolism
- Interferon-gamma/metabolism
- Interleukin-12/metabolism
- Interleukin-2 Receptor alpha Subunit/metabolism
- Lymphocyte Activation/immunology
- Mycobacterium bovis/immunology
- Receptors, Antigen, T-Cell, gamma-delta/analysis
- T-Lymphocyte Subsets/immunology
- Tuberculosis, Bovine/immunology
- Up-Regulation/immunology