Abstract / Description of output
Synchronized CV-1 cells were transfected with SV40 (simian virus 40) DNA-calcium phosphate co-precipitates. In the presence of carrier DNA, the transfection efficiency of SV40 DNA was decreased 5-fold in S-phase cells and was increased 4-fold in preparations of mitotically enriched cells as compared with asynchronous controls. No difference was observed when carrier DNA was omitted, when cells had progressed through S-phase and into G2-phase, or when the infectivity of cells to intact SV40 virus was tested. These results highlight the importance of cell-cycle-dependent factors on DNA-mediated gene transfer.
| Original language | English |
|---|---|
| Pages (from-to) | 529-33 |
| Number of pages | 5 |
| Journal | Biochemical Journal |
| Volume | 225 |
| Issue number | 2 |
| Publication status | Published - 15 Jan 1985 |
Keywords / Materials (for Non-textual outputs)
- Animals
- Cell Cycle
- Cell Line
- DNA
- DNA, Viral
- Hydroxyurea
- Mitosis
- Rats
- Simian virus 40
- Transfection