Enhancing the efficiency of transgene expression

A J Clark; A L Archibald; M McClenaghan; J P Simons; R Wallace; C B Whitelaw

doi:10.1098/rstb.1993.0020

Enhancing the efficiency of transgene expression

A J Clark
, A L Archibald
, M McClenaghan
, J P Simons
, R Wallace
, C B Whitelaw

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

Two strategies for enhancing gene expression in transgenic animals are described with particular reference to targeting expression to the mammary gland. Gene constructs in which the protein-encoding DNA sequences are contained within a genomic segment (comprising most or all of the natural introns of the corresponding gene) are shown to be expressed more efficiently than their intronless counterparts. Secondly, co-integrating an otherwise poorly expressed transgene in the vicinity of an actively expressed transgene can dramatically improve its efficiency of expression.

Original language	English
Pages (from-to)	225-32
Number of pages	8
Journal	Philosophical Transactions of the Royal Society B: Biological Sciences
Volume	339
Issue number	1288
DOIs	https://doi.org/10.1098/rstb.1993.0020
Publication status	Published - 27 Feb 1993

Keywords / Materials (for Non-textual outputs)

Animals
Female
Gene Expression
Genetic Techniques
Introns
Lactoglobulins
Mammary Glands, Animal
Mice
Mice, Transgenic
Transfection

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10.1098/rstb.1993.0020

Cite this

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abstract = "Two strategies for enhancing gene expression in transgenic animals are described with particular reference to targeting expression to the mammary gland. Gene constructs in which the protein-encoding DNA sequences are contained within a genomic segment (comprising most or all of the natural introns of the corresponding gene) are shown to be expressed more efficiently than their intronless counterparts. Secondly, co-integrating an otherwise poorly expressed transgene in the vicinity of an actively expressed transgene can dramatically improve its efficiency of expression.",

keywords = "Animals, Female, Gene Expression, Genetic Techniques, Introns, Lactoglobulins, Mammary Glands, Animal, Mice, Mice, Transgenic, Transfection",

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T1 - Enhancing the efficiency of transgene expression

AU - Clark, A J

AU - Archibald, A L

AU - McClenaghan, M

AU - Simons, J P

AU - Wallace, R

AU - Whitelaw, C B

PY - 1993/2/27

Y1 - 1993/2/27

N2 - Two strategies for enhancing gene expression in transgenic animals are described with particular reference to targeting expression to the mammary gland. Gene constructs in which the protein-encoding DNA sequences are contained within a genomic segment (comprising most or all of the natural introns of the corresponding gene) are shown to be expressed more efficiently than their intronless counterparts. Secondly, co-integrating an otherwise poorly expressed transgene in the vicinity of an actively expressed transgene can dramatically improve its efficiency of expression.

AB - Two strategies for enhancing gene expression in transgenic animals are described with particular reference to targeting expression to the mammary gland. Gene constructs in which the protein-encoding DNA sequences are contained within a genomic segment (comprising most or all of the natural introns of the corresponding gene) are shown to be expressed more efficiently than their intronless counterparts. Secondly, co-integrating an otherwise poorly expressed transgene in the vicinity of an actively expressed transgene can dramatically improve its efficiency of expression.

KW - Animals

KW - Female

KW - Gene Expression

KW - Genetic Techniques

KW - Introns

KW - Lactoglobulins

KW - Mammary Glands, Animal

KW - Mice

KW - Mice, Transgenic

KW - Transfection

U2 - 10.1098/rstb.1993.0020

DO - 10.1098/rstb.1993.0020

M3 - Article

C2 - 8097052

SN - 0962-8436

VL - 339

SP - 225

EP - 232

JO - Philosophical Transactions of the Royal Society B: Biological Sciences

JF - Philosophical Transactions of the Royal Society B: Biological Sciences

IS - 1288

ER -

Enhancing the efficiency of transgene expression

Abstract

Keywords / Materials (for Non-textual outputs)

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