Environmental distribution and abundance of the facultative methanotroph Methylocella

Md Tanvir Rahman, Andrew Crombie, Yin Chen, Nancy Stralis-Pavese, Levente Bodrossy, Patrick Meir, Niall P. McNamara, J. Colin Murrell

Research output: Contribution to journalArticlepeer-review

Abstract

Methylocella spp. are facultative methanotrophs, which are able to grow not only on methane but also on multicarbon substrates such as acetate, pyruvate or malate. Methylocella spp. were previously thought to be restricted to acidic soils such as peatlands, in which they may have a key role in methane oxidation. There is little information on the abundance and distribution of Methylocella spp. in the environment. New primers were designed, and a real-time quantitative PCR method was developed and validated targeting Methylocella mmoX (encoding the alpha-subunit of the soluble methane monooxygenase) that allowed the quantification of Methylocella spp. in environmental samples. We also developed and validated specific PCR assays, which target 16S rRNA genes of known Methylocella spp. These were used to investigate the distribution of Methylocella spp. in a variety of environmental samples. It was revealed that Methylocella species are widely distributed in nature and not restricted to acidic environments. The ISME Journal (2011) 5, 1061-1066; doi:10.1038/ismej.2010.190; published online 16 December 2010 Subject Category: microbial ecology and functional diversity of natural habitats

Original languageEnglish
Pages (from-to)1061-1066
Number of pages6
JournalThe ISME Journal
Volume5
Issue number6
DOIs
Publication statusPublished - Jun 2011

Keywords

  • distribution
  • abundance
  • facultative methanotroph
  • Methylocella
  • LANDFILL COVER SOIL
  • 16S RIBOSOMAL-RNA
  • SP NOV.
  • COMMUNITY STRUCTURE
  • ACIDIC PEATLANDS
  • PCR ASSAYS
  • BACTERIUM
  • PALUSTRIS
  • PEAT

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