Enzyme-Independent NO Stores in Human Skin: Quantification and Influence of UV Radiation

Megan Mowbray, Sara McLintock, Rebecca Weerakoon, Natalia Lomatschinsky, Sarah Jones, Adriano G Rossi, Richard B Weller

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Nitric oxide (NO) has many functions in the skin, including the mediation of inflammation and antimicrobial defense, wound healing, regulation of keratinocyte homeostasis, and regulation of apoptosis following UV radiation. NO is synthesized by a family of NO synthase enzymes, but its rapid release following UV exposure suggests the existence of preformed stores. NO can be converted into nitrite or nitrosothiols that are stable until cleaved by UV to release NO. Using dermal microdialysis, suction blister epidermal samples, and sweat collection, we demonstrated cutaneous concentrations of total NO-related products of 12 +/- 5.97 mu M, 0.03 +/- 0.03 mu mol mg(-1) epidermal protein, and 22 +/- 9.34 mu M, respectively. The predominant oxyanion was nitrate (60-75%) followed by nitrite. S-Nitrosothiols were barely detectable. Serum total NO-related products correlated directly with those of the upper dermis and sweat (R-2 = 0.62 and 0.3, respectively). UVA irradiation (10mW cm(-2)) increased the yield of NO-related products by microdialysis, peaking after 30 minutes. Dialysis with noradrenaline abrogated this rise. Both the skin and the dermal vasculature contain biologically significant stores of NO, particularly nitrite, which can be directly mobilized by UVA irradiation. The level of circulating NO-related products probably determines skin-bound stores.

Original languageEnglish
Pages (from-to)834-842
Number of pages9
JournalJournal of Investigative Dermatology
Volume129
Issue number4
DOIs
Publication statusPublished - Apr 2009

Keywords / Materials (for Non-textual outputs)

  • Ultraviolet Rays
  • Skin
  • Nitrates
  • Humans
  • Sweat
  • Vasoconstriction
  • S-Nitrosothiols
  • Nitric Oxide
  • Nitric Oxide Synthase
  • Nitrites
  • Adult
  • Saliva
  • Female
  • Male

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