EphB-ephrin-B2 interactions are required for thymus migration during organogenesis

Katie E. Foster, Julie Gordon, Kim Cardenas, Henrique Veiga-Fernandes, Taija Makinen, Elena Grigorieva, David G. Wilkinson, C. Clare Blackburn, Ellen Richie, Nancy R. Manley, Ralf H. Adams, Dimitris Kioussis, Mark C. Coles

Research output: Contribution to journalArticlepeer-review

Abstract

Thymus organogenesis requires coordinated interactions of multiple cell types, including neural crest (NC) cells, to orchestrate the formation, separation, and subsequent migration of the developing thymus from the third pharyngeal pouch to the thoracic cavity. The molecular mechanisms driving these processes are unclear; however, NC-derived mesenchyme has been shown to play an important role. Here, we show that, in the absence of ephrin-B2 expression on thymic NC-derived mesenchyme, the thymus remains in the cervical area instead of migrating into the thoracic cavity. Analysis of individual NC-derived thymic mesenchymal cells shows that, in the absence of ephrin-B2, their motility is impaired as a result of defective EphB receptor signaling. This implies a NC-derived cell-specific role of EphB-ephrin-B2 interactions in the collective migration of the thymic rudiment during organogenesis.

Original languageEnglish
Pages (from-to)13414-13419
Number of pages6
JournalProceedings of the National Academy of Sciences
Volume107
Issue number30
DOIs
Publication statusPublished - 27 Jul 2010

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