Epidermal growth factor signalling controls myosin II planar polarity to orchestrate convergent extension movements during Drosophila tubulogenesis

Aditya Saxena, Barry Denholm, Stephanie Bunt, Marcus Bischoff, Krishnaswamy VijayRaghavan, Helen Skaer

Research output: Contribution to journalArticlepeer-review

Abstract

Most epithelial tubes arise as small buds and elongate by regulated morphogenetic processes including oriented cell division, cell rearrangements, and changes in cell shape. Through live analysis of Drosophila renal tubule morphogenesis we show that tissue elongation results from polarised cell intercalations around the tubule circumference, producing convergent-extension tissue movements. Using genetic techniques, we demonstrate that the vector of cell movement is regulated by localised epidermal growth factor (EGF) signalling from the distally placed tip cell lineage, which sets up a distal-to-proximal gradient of pathway activation to planar polarise cells, without the involvement for PCP gene activity. Time-lapse imaging at subcellular resolution shows that the acquisition of planar polarity leads to asymmetric pulsatile Myosin II accumulation in the basal, proximal cortex of tubule cells, resulting in repeated, transient shortening of their circumferential length. This repeated bias in the polarity of cell contraction allows cells to move relative to each other, leading to a reduction in cell number around the lumen and an increase in tubule length. Physiological analysis demonstrates that animals whose tubules fail to elongate exhibit abnormal excretory function, defective osmoregulation, and lethality.

Original languageEnglish
Pages (from-to)e1002013
JournalPLoS Biology
Volume12
Issue number12
Early online date2 Dec 2014
DOIs
Publication statusPublished - 2 Dec 2014

Keywords

  • Animals
  • Cell Lineage
  • Cell Movement
  • Cell Polarity
  • Drosophila melanogaster
  • Embryo, Nonmammalian
  • Embryonic Development
  • Epidermal Growth Factor
  • Epithelium
  • Genes, Insect
  • Homeostasis
  • Malpighian Tubules
  • Models, Biological
  • Morphogenesis
  • Myosin Type II
  • Receptor, Epidermal Growth Factor
  • Signal Transduction

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