Epigenome-wide association study of leukocyte telomere length

Yunsung Lee, Dianjianyi Sun, Anil P. S. Ori, Ake T Lu, Anne Seeboth, Sarah Harris, Ian Deary, Riccardo Marioni, Mette Soerensen, Jonas Mengel-From, Jacob Hjelmborg, Kaare Christensen, James G. Wilson, Daniel Levy, Alex P Reiner, Wei Chen, Shengxu Li, Jennifer R. Harris, Per Magnus, Abraham AvivAstanand Jugessur, Steve Horvath

Research output: Contribution to journalArticlepeer-review


Telomere length is associated with age-related diseases and is highly heritable. It is unclear, however, to what extent epigenetic modifications are associated with leukocyte telomere length (LTL). In this study, we conducted a large-scale epigenome-wide association study (EWAS) of LTL using seven large cohorts (n=5,713) - the Framingham Heart Study, the Jackson Heart Study, the Women's Health Initiative, the Bogalusa Heart Study, the Lothian Birth Cohorts of 1921 and 1936, and the Longitudinal Study of Aging Danish Twins. Our stratified analysis suggests that EWAS findings for women of African ancestry may be distinct from those of three other groups: males of African ancestry, and males and females of European ancestry. Using a meta-analysis framework, we identified DNA methylation (DNAm) levels at 823 CpG sites to be significantly associated (P<1E-7) with LTL after adjusting for age, sex, ethnicity, and imputed white blood cell counts. Functional enrichment analyses revealed that these CpG sites are near genes that play a role in circadian rhythm, blood coagulation, and wound healing. Weighted correlation network analysis identified four co-methylation modules associated with LTL, age, and blood cell counts. Overall, this study reveals highly significant relationships between two hallmarks of aging: telomere biology and epigenetic changes.
Original languageEnglish
Early online date26 Aug 2019
Publication statusE-pub ahead of print - 26 Aug 2019

Fingerprint Dive into the research topics of 'Epigenome-wide association study of leukocyte telomere length'. Together they form a unique fingerprint.

Cite this