Projects per year
Objectives: To determine (a) whether botulism causes autonomic and enteric neurodegeneration, and (b) the effect of EGS on the expression of SNARE proteins within cranial cervical ganglion [CCG] and enteric neuronal perikarya.
Methods: Light microscopy was used to compare the morphology of neurons in haematoxylin-eosin stained sections of CCG and ileum from 6 EGS horses, 5 botulism horses and 6 control horses. Immunohistochemistry was used to compare the expression of synaptosomal-associated protein-25 (SNAP-25), synaptobrevin (Syb) and syntaxin (Syn) within CCG neurons, and of Syb in enteric neurons, from horses with EGS, horses with botulism and control horses. The concentrations of these SNARE proteins in extracts of CCG from EGS and control horses were compared using quantitative fluorescent western blotting (QFWB).
Results: EGS, but not botulism, was associated with autonomic and enteric neurodegeneration and with increased immunoreactivity for SNARE proteins within neuronal perikarya. QFWB confirmed increased concentrations of SNAP-25, Syb and Syn within CCG extracts from EGS versus control horses, with the increases in the latter two proteins being statistically significant.
Conclusions and potential relevance: The occurrence of autonomic and enteric neurodegeneration, and increased expression of SNARE proteins within neuronal perikarya, in EGS but not botulism, suggests that EGS may not be caused by BoNTs. Further investigation of the aetiology of EGS is therefore warranted.
- grass sickness
- SNARE proteins
1/04/02 → 31/03/11