Estimating prion concentration in fluids and tissues by quantitative PMCA

Baian Chen; Rodrigo Morales; Marcelo A Barria; Claudio Soto

doi:10.1038/nmeth.1465

Estimating prion concentration in fluids and tissues by quantitative PMCA

Baian Chen, Rodrigo Morales, Marcelo A Barria, Claudio Soto

Deanery of Clinical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract / Description of output

Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrP(Sc)) in brain, spleen, blood and urine of scrapie-affected hamsters.

Original language	English
Pages (from-to)	519-20
Number of pages	2
Journal	Nature Methods
Volume	7
Issue number	7
DOIs	https://doi.org/10.1038/nmeth.1465
Publication status	Published - Jul 2010

Keywords / Materials (for Non-textual outputs)

Animals
Cricetinae
Mesocricetus
PrPSc Proteins
Protein Folding
Scrapie

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10.1038/nmeth.1465

Cite this

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title = "Estimating prion concentration in fluids and tissues by quantitative PMCA",

abstract = "Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrP(Sc)) in brain, spleen, blood and urine of scrapie-affected hamsters.",

keywords = "Animals, Cricetinae, Mesocricetus, PrPSc Proteins, Protein Folding, Scrapie",

author = "Baian Chen and Rodrigo Morales and Barria, {Marcelo A} and Claudio Soto",

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journal = "Nature Methods",

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T1 - Estimating prion concentration in fluids and tissues by quantitative PMCA

AU - Chen, Baian

AU - Morales, Rodrigo

AU - Barria, Marcelo A

AU - Soto, Claudio

PY - 2010/7

Y1 - 2010/7

N2 - Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrP(Sc)) in brain, spleen, blood and urine of scrapie-affected hamsters.

AB - Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrP(Sc)) in brain, spleen, blood and urine of scrapie-affected hamsters.

KW - Animals

KW - Cricetinae

KW - Mesocricetus

KW - PrPSc Proteins

KW - Protein Folding

KW - Scrapie

U2 - 10.1038/nmeth.1465

DO - 10.1038/nmeth.1465

M3 - Article

C2 - 20512142

SN - 1548-7091

VL - 7

SP - 519

EP - 520

JO - Nature Methods

JF - Nature Methods

IS - 7

ER -

Estimating prion concentration in fluids and tissues by quantitative PMCA

Abstract / Description of output

Keywords / Materials (for Non-textual outputs)

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