Estimating prion concentration in fluids and tissues by quantitative PMCA

Baian Chen, Rodrigo Morales, Marcelo A Barria, Claudio Soto

Research output: Contribution to journalArticlepeer-review

Abstract

Prions, the proteinaceous infectious agent responsible for prion diseases, can be detected with high sensitivity by protein misfolding cyclic amplification (PMCA) technology. Here we describe a quantitative PMCA procedure to calculate the concentration of very low levels of prions in biological samples. Using this procedure, we determined the quantities of misfolded prion protein (PrP(Sc)) in brain, spleen, blood and urine of scrapie-affected hamsters.

Original languageEnglish
Pages (from-to)519-20
Number of pages2
JournalNature Methods
Volume7
Issue number7
DOIs
Publication statusPublished - Jul 2010

Keywords

  • Animals
  • Cricetinae
  • Mesocricetus
  • PrPSc Proteins
  • Protein Folding
  • Scrapie

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