Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection.

Karsten  Becker; A. R. Larsen; Robert L. Skov; Gavin Paterson; Mark A. Holmes; AJ Sabat; AW  Friedrich; R. Kock; G B Peters; André Kriegeskorte

doi:10.1128/JCM.00075-13

Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection.

Karsten Becker, A. R. Larsen, Robert L. Skov, Gavin Paterson, Mark A. Holmes, AJ Sabat, AW Friedrich, R. Kock, G B Peters, André Kriegeskorte

Royal (Dick) School of Veterinary Studies

Research output: Contribution to journal › Article › peer-review

Abstract

A mecC (mecA LGA251)-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA- and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

Original language	English
Pages (from-to)	1917-9
Journal	Journal of Clinical Microbiology
Volume	51
Issue number	6
Early online date	20 Mar 2013
DOIs	https://doi.org/10.1128/JCM.00075-13
Publication status	Published - 16 May 2013

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abstract = "A mecC (mecA LGA251)-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA- and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.",

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AU - Becker, Karsten

AU - Larsen, A. R.

AU - Skov, Robert L.

AU - Paterson, Gavin

AU - Holmes, Mark A.

AU - Sabat, AJ

AU - Friedrich, AW

AU - Kock, R.

AU - Peters, G B

AU - Kriegeskorte, André

PY - 2013/5/16

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AB - A mecC (mecA LGA251)-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA- and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.

U2 - 10.1128/JCM.00075-13

DO - 10.1128/JCM.00075-13

M3 - Article

VL - 51

SP - 1917

EP - 1919

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 6

ER -

Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection.

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