Evaluation of an enzyme immunoassay for antibodies to a recombinant Blastomyces adhesin-1 repeat antigen as an aid in the diagnosis of blastomycosis in dogs

Alyssa C Mourning, Edward E Patterson, Emily J Kirsch, Janelle S Renschler, Linda A Wolf, Jasmin K Paris, Michelle M Durkin, Lawrence J Wheat

Research output: Contribution to journalArticlepeer-review

Abstract

OBJECTIVE: To evaluate the sensitivity and specificity of an enzyme immunoassay (EIA) for antibodies to a recombinant Blastomyces adhesin-1 repeat antigen (rBAD-1) to aid in the diagnosis of blastomycosis in dogs and compare the findings with results from other tests used for this purpose.

DESIGN: Prospective analytic study.

SAMPLE: Serum and urine from 70 dogs with and without blastomycosis.

PROCEDURES: Serum and urine samples were collected from dogs with blastomycosis (n = 21), histoplasmosis (8), or nonfungal pulmonary disease (21) and from healthy control dogs living in a blastomycosis-endemic area (20). Serum was tested for antibodies against Blastomyces dermatitidis with the rBAD-1 antibody EIA and an A-antigen antibody agar gel immunodiffusion (AGID) assay. Serum and urine were tested for B dermatitidis antigen with a quantitative EIA.

RESULTS: Sensitivity of the quantitative antigen EIA was 100% in serum and urine samples from dogs with blastomycosis, with specificity of 95% in urine samples from dogs with nonfungal pulmonary disease and 100% in urine samples from healthy dogs. Sensitivity of the rBAD-1 antibody EIA (95%) was significantly greater than that of the A-antigen antibody AGID assay (65%). Specificity of the antibody EIA was 88% in dogs with histoplasmosis, 95% in healthy dogs, and 100% in dogs with nonfungal pulmonary disease.

CONCLUSIONS AND CLINICAL RELEVANCE: The rBAD-1 antibody EIA had greater sensitivity than the A-antigen antibody AGID assay in dogs with blastomycosis. This antibody EIA may assist in distinguishing histoplasmosis from blastomycosis. Further evaluation in a larger prospective study is needed to verify these results.

Original languageEnglish
Pages (from-to)1133-8
Number of pages6
JournalJournal of the American Veterinary Medical Association
Volume247
Issue number10
DOIs
Publication statusPublished - 15 Nov 2015

Keywords

  • Animals
  • Antibodies, Fungal/blood
  • Antigens, Fungal/immunology
  • Blastomyces/metabolism
  • Blastomycosis/blood
  • Dog Diseases/diagnosis
  • Dogs
  • Female
  • Immunoenzyme Techniques/methods
  • Male
  • Sensitivity and Specificity

Fingerprint

Dive into the research topics of 'Evaluation of an enzyme immunoassay for antibodies to a recombinant Blastomyces adhesin-1 repeat antigen as an aid in the diagnosis of blastomycosis in dogs'. Together they form a unique fingerprint.

Cite this