Evaluation of antibody serology to determine current helminth and plasmodium falciparum infections in a co-endemic area in Southern Mozambique

Rebeca Santano; Rocío Rubio; Berta Grau-Pujol; Valdemiro Escola; Osvaldo Muchisse; Inocência Cuamba; Marta Vidal; Gemma Ruiz-Olalla; Ruth Aguilar; Javier Gandasegui; Maria Demontis; Jose Carlos Jamine; Anélsio Cossa; Charfudin Sacoor; Jorge Cano; Luis Izquierdo; Chetan E. Chitnis; Ross L. Coppel; Virander Chauhan; David Cavanagh; Sheetij Dutta; Evelina Angov; Lisette Van Lieshout; Bin Zhan; José Muñoz; Carlota Dobaño; Gemma Moncunill; Siddhartha Mahanty

doi:10.1371/journal.pntd.0010138

Evaluation of antibody serology to determine current helminth and plasmodium falciparum infections in a co-endemic area in Southern Mozambique

Rebeca Santano, Rocío Rubio, Berta Grau-Pujol, Valdemiro Escola, Osvaldo Muchisse, Inocência Cuamba, Marta Vidal, Gemma Ruiz-Olalla, Ruth Aguilar, Javier Gandasegui, Maria Demontis, Jose Carlos Jamine, Anélsio Cossa, Charfudin Sacoor, Jorge Cano, Luis Izquierdo, Chetan E. Chitnis, Ross L. Coppel, Virander Chauhan, David CavanaghSheetij Dutta, Evelina Angov, Lisette Van Lieshout, Bin Zhan, José Muñoz, Carlota Dobaño, Gemma Moncunill, Siddhartha Mahanty (Editor)

School of Biological Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Background
Soil-transmitted helminths (STH), Schistosoma spp. and Plasmodium falciparum are parasites of major public health importance and co-endemic in many sub-Saharan African countries. Management of these infections requires detection and treatment of infected people and evaluation of large-scale measures implemented. Diagnostic tools are available but their low sensitivity, especially for low intensity helminth infections, leaves room for improvement. Antibody serology could be a useful approach thanks to its potential to detect both current infection and past exposure.

Methodology
We evaluated total IgE responses and specific-IgG levels to 9 antigens from STH, 2 from Schistosoma spp., and 16 from P. falciparum, as potential markers of current infection in a population of children and adults from Southern Mozambique (N = 715). Antibody responses were measured by quantitative suspension array Luminex technology and their performance was evaluated by ROC curve analysis using microscopic and molecular detection of infections as reference.

Principal findings
IgG against the combination of EXP1, AMA1 and MSP2 (P. falciparum) in children and NIE (Strongyloides stercoralis) in adults and children had the highest accuracies (AUC = 0.942 and AUC = 0.872, respectively) as markers of current infection. IgG against the combination of MEA and Sm25 (Schistosoma spp.) were also reliable markers of current infection (AUC = 0.779). In addition, IgG seropositivity against 20 out of the 27 antigens in the panel differentiated the seropositive endemic population from the non-endemic population, suggesting a possible role as markers of exposure although sensitivity could not be assessed.

Conclusions
We provided evidence for the utility of antibody serology to detect current infection with parasites causing tropical diseases in endemic populations. In addition, most of the markers have potential good specificity as markers of exposure. We also showed the feasibility of measuring antibody serology with a platform that allows the integration of control and elimination programs for different pathogens.

Original language	English
Article number	e0010138
Number of pages	30
Journal	PLoS Neglected Tropical Diseases
Volume	16
Issue number	6
DOIs	https://doi.org/10.1371/journal.pntd.0010138
Publication status	Published - 21 Jun 2022

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Santano, R., Rubio, R., Grau-Pujol, B., Escola, V., Muchisse, O., Cuamba, I., Vidal, M., Ruiz-Olalla, G., Aguilar, R., Gandasegui, J., Demontis, M., Jamine, J. C., Cossa, A., Sacoor, C., Cano, J., Izquierdo, L., Chitnis, C. E., Coppel, R. L., Chauhan, V., ... Mahanty, S. (Ed.) (2022). Evaluation of antibody serology to determine current helminth and plasmodium falciparum infections in a co-endemic area in Southern Mozambique. PLoS Neglected Tropical Diseases, 16(6), Article e0010138. https://doi.org/10.1371/journal.pntd.0010138

@article{1839de53b17a47649ecf17adbe1b6f69,

title = "Evaluation of antibody serology to determine current helminth and plasmodium falciparum infections in a co-endemic area in Southern Mozambique",

abstract = "Background Soil-transmitted helminths (STH), Schistosoma spp. and Plasmodium falciparum are parasites of major public health importance and co-endemic in many sub-Saharan African countries. Management of these infections requires detection and treatment of infected people and evaluation of large-scale measures implemented. Diagnostic tools are available but their low sensitivity, especially for low intensity helminth infections, leaves room for improvement. Antibody serology could be a useful approach thanks to its potential to detect both current infection and past exposure. Methodology We evaluated total IgE responses and specific-IgG levels to 9 antigens from STH, 2 from Schistosoma spp., and 16 from P. falciparum, as potential markers of current infection in a population of children and adults from Southern Mozambique (N = 715). Antibody responses were measured by quantitative suspension array Luminex technology and their performance was evaluated by ROC curve analysis using microscopic and molecular detection of infections as reference. Principal findings IgG against the combination of EXP1, AMA1 and MSP2 (P. falciparum) in children and NIE (Strongyloides stercoralis) in adults and children had the highest accuracies (AUC = 0.942 and AUC = 0.872, respectively) as markers of current infection. IgG against the combination of MEA and Sm25 (Schistosoma spp.) were also reliable markers of current infection (AUC = 0.779). In addition, IgG seropositivity against 20 out of the 27 antigens in the panel differentiated the seropositive endemic population from the non-endemic population, suggesting a possible role as markers of exposure although sensitivity could not be assessed. Conclusions We provided evidence for the utility of antibody serology to detect current infection with parasites causing tropical diseases in endemic populations. In addition, most of the markers have potential good specificity as markers of exposure. We also showed the feasibility of measuring antibody serology with a platform that allows the integration of control and elimination programs for different pathogens.",

author = "Rebeca Santano and Roc{\'i}o Rubio and Berta Grau-Pujol and Valdemiro Escola and Osvaldo Muchisse and Inoc{\^e}ncia Cuamba and Marta Vidal and Gemma Ruiz-Olalla and Ruth Aguilar and Javier Gandasegui and Maria Demontis and Jamine, \{Jose Carlos\} and An{\'e}lsio Cossa and Charfudin Sacoor and Jorge Cano and Luis Izquierdo and Chitnis, \{Chetan E.\} and Coppel, \{Ross L.\} and Virander Chauhan and David Cavanagh and Sheetij Dutta and Evelina Angov and \{Van Lieshout\}, Lisette and Bin Zhan and Jos{\'e} Mu{\~n}oz and Carlota Doba{\~n}o and Gemma Moncunill and Siddhartha Mahanty",

year = "2022",

month = jun,

day = "21",

doi = "10.1371/journal.pntd.0010138",

language = "English",

volume = "16",

journal = "PLoS Neglected Tropical Diseases",

issn = "1935-2727",

publisher = "Public Library of Science",

number = "6",

}

Santano, R, Rubio, R, Grau-Pujol, B, Escola, V, Muchisse, O, Cuamba, I, Vidal, M, Ruiz-Olalla, G, Aguilar, R, Gandasegui, J, Demontis, M, Jamine, JC, Cossa, A, Sacoor, C, Cano, J, Izquierdo, L, Chitnis, CE, Coppel, RL, Chauhan, V, Cavanagh, D, Dutta, S, Angov, E, Van Lieshout, L, Zhan, B, Muñoz, J, Dobaño, C, Moncunill, G & Mahanty, S (ed.) 2022, 'Evaluation of antibody serology to determine current helminth and plasmodium falciparum infections in a co-endemic area in Southern Mozambique', PLoS Neglected Tropical Diseases, vol. 16, no. 6, e0010138. https://doi.org/10.1371/journal.pntd.0010138

TY - JOUR

T1 - Evaluation of antibody serology to determine current helminth and plasmodium falciparum infections in a co-endemic area in Southern Mozambique

AU - Santano, Rebeca

AU - Rubio, Rocío

AU - Grau-Pujol, Berta

AU - Escola, Valdemiro

AU - Muchisse, Osvaldo

AU - Cuamba, Inocência

AU - Vidal, Marta

AU - Ruiz-Olalla, Gemma

AU - Aguilar, Ruth

AU - Gandasegui, Javier

AU - Demontis, Maria

AU - Jamine, Jose Carlos

AU - Cossa, Anélsio

AU - Sacoor, Charfudin

AU - Cano, Jorge

AU - Izquierdo, Luis

AU - Chitnis, Chetan E.

AU - Coppel, Ross L.

AU - Chauhan, Virander

AU - Cavanagh, David

AU - Dutta, Sheetij

AU - Angov, Evelina

AU - Van Lieshout, Lisette

AU - Zhan, Bin

AU - Muñoz, José

AU - Dobaño, Carlota

AU - Moncunill, Gemma

A2 - Mahanty, Siddhartha

PY - 2022/6/21

Y1 - 2022/6/21

N2 - Background Soil-transmitted helminths (STH), Schistosoma spp. and Plasmodium falciparum are parasites of major public health importance and co-endemic in many sub-Saharan African countries. Management of these infections requires detection and treatment of infected people and evaluation of large-scale measures implemented. Diagnostic tools are available but their low sensitivity, especially for low intensity helminth infections, leaves room for improvement. Antibody serology could be a useful approach thanks to its potential to detect both current infection and past exposure. Methodology We evaluated total IgE responses and specific-IgG levels to 9 antigens from STH, 2 from Schistosoma spp., and 16 from P. falciparum, as potential markers of current infection in a population of children and adults from Southern Mozambique (N = 715). Antibody responses were measured by quantitative suspension array Luminex technology and their performance was evaluated by ROC curve analysis using microscopic and molecular detection of infections as reference. Principal findings IgG against the combination of EXP1, AMA1 and MSP2 (P. falciparum) in children and NIE (Strongyloides stercoralis) in adults and children had the highest accuracies (AUC = 0.942 and AUC = 0.872, respectively) as markers of current infection. IgG against the combination of MEA and Sm25 (Schistosoma spp.) were also reliable markers of current infection (AUC = 0.779). In addition, IgG seropositivity against 20 out of the 27 antigens in the panel differentiated the seropositive endemic population from the non-endemic population, suggesting a possible role as markers of exposure although sensitivity could not be assessed. Conclusions We provided evidence for the utility of antibody serology to detect current infection with parasites causing tropical diseases in endemic populations. In addition, most of the markers have potential good specificity as markers of exposure. We also showed the feasibility of measuring antibody serology with a platform that allows the integration of control and elimination programs for different pathogens.

AB - Background Soil-transmitted helminths (STH), Schistosoma spp. and Plasmodium falciparum are parasites of major public health importance and co-endemic in many sub-Saharan African countries. Management of these infections requires detection and treatment of infected people and evaluation of large-scale measures implemented. Diagnostic tools are available but their low sensitivity, especially for low intensity helminth infections, leaves room for improvement. Antibody serology could be a useful approach thanks to its potential to detect both current infection and past exposure. Methodology We evaluated total IgE responses and specific-IgG levels to 9 antigens from STH, 2 from Schistosoma spp., and 16 from P. falciparum, as potential markers of current infection in a population of children and adults from Southern Mozambique (N = 715). Antibody responses were measured by quantitative suspension array Luminex technology and their performance was evaluated by ROC curve analysis using microscopic and molecular detection of infections as reference. Principal findings IgG against the combination of EXP1, AMA1 and MSP2 (P. falciparum) in children and NIE (Strongyloides stercoralis) in adults and children had the highest accuracies (AUC = 0.942 and AUC = 0.872, respectively) as markers of current infection. IgG against the combination of MEA and Sm25 (Schistosoma spp.) were also reliable markers of current infection (AUC = 0.779). In addition, IgG seropositivity against 20 out of the 27 antigens in the panel differentiated the seropositive endemic population from the non-endemic population, suggesting a possible role as markers of exposure although sensitivity could not be assessed. Conclusions We provided evidence for the utility of antibody serology to detect current infection with parasites causing tropical diseases in endemic populations. In addition, most of the markers have potential good specificity as markers of exposure. We also showed the feasibility of measuring antibody serology with a platform that allows the integration of control and elimination programs for different pathogens.

U2 - 10.1371/journal.pntd.0010138

DO - 10.1371/journal.pntd.0010138

M3 - Article

SN - 1935-2727

VL - 16

JO - PLoS Neglected Tropical Diseases

JF - PLoS Neglected Tropical Diseases

IS - 6

M1 - e0010138

ER -

Evaluation of antibody serology to determine current helminth and plasmodium falciparum infections in a co-endemic area in Southern Mozambique

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