EVALUATION OF LOCAL ENDOBRONCHIAL ANTIGEN CHALLENGES IN THE INVESTIGATION OF EQUINE CHRONIC OBSTRUCTIVE PULMONARY-DISEASE

Bruce McGorum, Padraic Dixon

Research output: Contribution to journalArticlepeer-review

Abstract

Local transendoscopic endobronchial antigen challenge, which has proved to be a valuable clinical and research technique in the study of human pulmonary hypersensitivity, was evaluated in control and asymptomatic COPD - affected horses.

Transendoscopic endobronchial challenges with phosphate-buffered saline (PBS), Micropolyspora faeni extract at 60 and 600 mug/ml and mouldy hav extract elicited neutrophilic airway inflammatory responses in control (N=5-7) and asymptomatic COPD-affected (N=5-7) horses, as determined by cytological examinations of bronchoalveolar lavage fluid (BALF) harvested from the challenged lung segments.

Endobronchial challenges with 600 mug M. faeni extract/ml induced a significant BALF neutrophilia only in horses with asymptomatic COPD, when compared with PBS challenges. However, as the BALF neutrophil ratios of COPD-affected horses after this M. faeni challenge did not differ significantly from those of control horses, this finding has little clinical diagnostic value. The BALF neutrophilia induced in control and asymptomatic COPD-affected horses by 60 mug M. faeni extract/ml and mouldy hay extract challenges was not significantly different from that induced by PBS challenge.

Endoscopically visible bronchial changes were observed in some of the control and COPD-affected horses within 5 mins and at 5 h after PBS, M. faeni and mouldy hay extract challenges.

We conclude that this technique is of no value in the investigation of equine COPD.

Original languageEnglish
Pages (from-to)269-272
Number of pages4
JournalEquine Veterinary Journal
Volume25
Issue number4
Publication statusPublished - Jul 1993

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