Evaluation of N-glycan-decorated live attenuated escherichia coli and outer membrane vesicles as vaccines against campylobacter jejuni colonisation in chickens

Glycoengineering of Veterinary Vaccines consortium (GoVV)

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Campylobacter jejuni is a leading global cause of bacterial gastroenteritis in humans, and poultry are a major reservoir. Glycoconjugate vaccines containing the conserved C. jejuni N-glycan have previously been reported to be effective at reducing caecal colonisation of chickens by C. jejuni. These include recombinant subunit vaccines, live E. coli strains expressing the N-glycan on the surface as well as outer membrane vesicles (OMVs) derived from these E. coli strains. In this study, we evaluated the efficacy of live E. coli expressing the C. jejuni N-glycan from a plasmid and glycosylated OMVs (G-OMVs) derived from them against colonisation by different C. jejuni strains. Despite the C. jejuni N-glycan being expressed on the surface of the live strain and the OMVs, no reduction in caecal colonisation by C. jejuni was observed and N-glycan-specific responses were not detected.
Original languageEnglish
Number of pages7
JournalVaccine
Volume41
Issue number29
Early online date3 Jun 2023
DOIs
Publication statusPublished - 29 Jun 2023

Keywords / Materials (for Non-textual outputs)

  • glycoconjugate
  • vaccine
  • campylobacter
  • colonisation
  • chickens

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