Abstract / Description of output
Objective: To examine the accuracy of using body fluids macroscopically suspected to contain erythrocytes to determine the blood type in dogs and cats by use of an immunochromatographic cartridge (ICC), compared to systemic blood as the reference standard.
Design: Prospective study.
Setting: University teaching hospital.
Animals: Thirty client-owned dogs and 8 cats.
Interventions: Dogs and cats with a sanguineous or serosanguineous body fluid (SBF) who also required a blood sample, were eligible for inclusion. Packed cell volume and blood type were determined in all blood and fluid samples. For body fluids with a low PCV and discordant blood type results compared to systemic blood, sample concentration and repeat blood typing from the fluid was performed when enough sample was available.
Measurement and main results: Body fluid samples consisted of 16 pleural (11 dogs; 5 cats), 12 peritoneal (10 dogs; 2 cats) and 4 canine pericardial effusions, 3 urine samples and one each of feces and epistaxis from dogs and a seroma sample from a cat. Median manual PCV of blood and fluid samples were 34% (14 – 66%) and 6% (0.5 – 70%) for dogs and 28% (14 – 48%) and 14% (0.5 – 19%) for cats, respectively. Dogs correctly classified as being DEA 1 negative, DEA 1 positive and DEA 1 weak positive when using body fluid to blood type were 13/14, 4/9 and 5/7, respectively. All reference blood type to fluid blood type (FBT) discordant results had a body fluid PCV equal to or below 2%. Subsequently concentrated body fluid samples had a PCV above 8% and repeat FBT matched RBT. All cats were classified as type A by all RBTs and FBTs.
Conclusions: Body fluids containing erythrocytes may be utilized to blood type dogs if sufficiently concentrated and to blood type A cats.
Design: Prospective study.
Setting: University teaching hospital.
Animals: Thirty client-owned dogs and 8 cats.
Interventions: Dogs and cats with a sanguineous or serosanguineous body fluid (SBF) who also required a blood sample, were eligible for inclusion. Packed cell volume and blood type were determined in all blood and fluid samples. For body fluids with a low PCV and discordant blood type results compared to systemic blood, sample concentration and repeat blood typing from the fluid was performed when enough sample was available.
Measurement and main results: Body fluid samples consisted of 16 pleural (11 dogs; 5 cats), 12 peritoneal (10 dogs; 2 cats) and 4 canine pericardial effusions, 3 urine samples and one each of feces and epistaxis from dogs and a seroma sample from a cat. Median manual PCV of blood and fluid samples were 34% (14 – 66%) and 6% (0.5 – 70%) for dogs and 28% (14 – 48%) and 14% (0.5 – 19%) for cats, respectively. Dogs correctly classified as being DEA 1 negative, DEA 1 positive and DEA 1 weak positive when using body fluid to blood type were 13/14, 4/9 and 5/7, respectively. All reference blood type to fluid blood type (FBT) discordant results had a body fluid PCV equal to or below 2%. Subsequently concentrated body fluid samples had a PCV above 8% and repeat FBT matched RBT. All cats were classified as type A by all RBTs and FBTs.
Conclusions: Body fluids containing erythrocytes may be utilized to blood type dogs if sufficiently concentrated and to blood type A cats.
| Original language | English |
|---|---|
| Pages | S2-S41 |
| Number of pages | 1 |
| DOIs | |
| Publication status | Published - 11 Apr 2022 |
| Event | International Veterinary Emergency and Critical Care Symposium, and the European Veterinary Emergency and Critical Care Annual Congress 2021 - Nashville, United States Duration: 11 Sept 2021 → 15 Sept 2021 |
Conference
| Conference | International Veterinary Emergency and Critical Care Symposium, and the European Veterinary Emergency and Critical Care Annual Congress 2021 |
|---|---|
| Country/Territory | United States |
| City | Nashville |
| Period | 11/09/21 → 15/09/21 |