Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR

David Bonsall, William F Gregory, Camilla L C Ip, Sharyne Donfield, James Iles, M Azim Ansari, Paolo Piazza, Amy Trebes, Anthony Brown, John Frater, Oliver G Pybus, Phillip Goulder, Paul Klenerman, Rory Bowden, Edward D Gomperts, Eleanor Barnes, Amit Kapoor, Colin P Sharp, Peter Simmonds

Research output: Contribution to journalArticlepeer-review

Abstract

Next-generation sequencing has critical applications in virus discovery, diagnostics, and environmental surveillance. We used metagenomic sequence libraries for retrospective screening of plasma samples for the recently discovered human hepegivirus 1 (HHpgV-1). From a cohort of 150 hepatitis C virus (HCV)-positive case-patients, we identified 2 persons with HHpgV-1 viremia and a high frequency of human pegivirus (HPgV) viremia (14%). Detection of HHpgV-1 and HPgV was concordant with parallel PCR-based screening using conserved primers matching groups 1 (HPgV) and 2 (HHPgV-1) nonstructural 3 region sequences. PCR identified 1 HHPgV-1-positive person with viremia from a group of 195 persons with hemophilia who had been exposed to nonvirally inactivated factor VII/IX; 18 (9%) were HPgV-positive. Relative to HCV and HPgV, active infections with HHpgV-1 were infrequently detected in blood, even in groups that had substantial parenteral exposure. Our findings are consistent with lower transmissibility or higher rates of virus clearance for HHpgV-1 than for other bloodborne human flaviviruses.

Original languageEnglish
Pages (from-to)671-678
Number of pages8
JournalEmerging Infectious Diseases
Volume22
Issue number4
DOIs
Publication statusPublished - Apr 2016

Fingerprint Dive into the research topics of 'Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR'. Together they form a unique fingerprint.

Cite this