Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR

David Bonsall; William F Gregory; Camilla L C Ip; Sharyne Donfield; James Iles; M Azim Ansari; Paolo Piazza; Amy Trebes; Anthony Brown; John Frater; Oliver G Pybus; Phillip Goulder; Paul Klenerman; Rory Bowden; Edward D Gomperts; Eleanor Barnes; Amit Kapoor; Colin P Sharp; Peter Simmonds

doi:10.3201/eid2204.151812

Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR

David Bonsall, William F Gregory, Camilla L C Ip, Sharyne Donfield, James Iles, M Azim Ansari, Paolo Piazza, Amy Trebes, Anthony Brown, John Frater, Oliver G Pybus, Phillip Goulder, Paul Klenerman, Rory Bowden, Edward D Gomperts, Eleanor Barnes, Amit Kapoor, Colin P Sharp, Peter Simmonds

Research output: Contribution to journal › Article › peer-review

Abstract

Next-generation sequencing has critical applications in virus discovery, diagnostics, and environmental surveillance. We used metagenomic sequence libraries for retrospective screening of plasma samples for the recently discovered human hepegivirus 1 (HHpgV-1). From a cohort of 150 hepatitis C virus (HCV)-positive case-patients, we identified 2 persons with HHpgV-1 viremia and a high frequency of human pegivirus (HPgV) viremia (14%). Detection of HHpgV-1 and HPgV was concordant with parallel PCR-based screening using conserved primers matching groups 1 (HPgV) and 2 (HHPgV-1) nonstructural 3 region sequences. PCR identified 1 HHPgV-1-positive person with viremia from a group of 195 persons with hemophilia who had been exposed to nonvirally inactivated factor VII/IX; 18 (9%) were HPgV-positive. Relative to HCV and HPgV, active infections with HHpgV-1 were infrequently detected in blood, even in groups that had substantial parenteral exposure. Our findings are consistent with lower transmissibility or higher rates of virus clearance for HHpgV-1 than for other bloodborne human flaviviruses.

Original language	English
Pages (from-to)	671-678
Number of pages	8
Journal	Emerging Infectious Diseases
Volume	22
Issue number	4
DOIs	https://doi.org/10.3201/eid2204.151812
Publication status	Published - Apr 2016

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10.3201/eid2204.151812

Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR
Copyright Emerging Infectious Diseases is published by the Centers for Disease Control and Prevention, a U.S. Government agency. Therefore, all materials published in Emerging Infectious Diseases are in the public domain and can be used without permission. Proper citation, however, is required.
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http://wwwnc.cdc.gov/eid/article/22/4/15-1812_article

ISP4 Pathogenesis and resistance in viral diseases of livestock
Dutia, B. (Principal Investigator), Archibald, A. (Co-investigator), Beard, P. (Co-investigator), Bishop, S. (Co-investigator), Bronsvoort, M. (Co-investigator), Burt, D. (Co-investigator), Collie, D. (Co-investigator), Digard, P. (Co-investigator), Freeman, T. (Co-investigator), Glass, E. (Co-investigator), Grey, F. (Co-investigator), Hocking, P. (Co-investigator), Houston, R. (Co-investigator), Hume, D. (Co-investigator), Kaiser, P. (Co-investigator), Nash, A. (Co-investigator), Sang, H. (Co-investigator), Sharp, C. (Co-investigator), Watson, M. (Co-investigator) & Whitelaw, B. (Co-investigator)
Biotechnology and Biological Sciences Research Council
1/04/12 → 31/03/17
Project: Research

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Bonsall, D., Gregory, W. F., Ip, C. L. C., Donfield, S., Iles, J., Ansari, M. A., Piazza, P., Trebes, A., Brown, A., Frater, J., Pybus, O. G., Goulder, P., Klenerman, P., Bowden, R., Gomperts, E. D., Barnes, E., Kapoor, A., Sharp, C. P., & Simmonds, P. (2016). Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR. Emerging Infectious Diseases, 22(4), 671-678. https://doi.org/10.3201/eid2204.151812

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title = "Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR",

abstract = "Next-generation sequencing has critical applications in virus discovery, diagnostics, and environmental surveillance. We used metagenomic sequence libraries for retrospective screening of plasma samples for the recently discovered human hepegivirus 1 (HHpgV-1). From a cohort of 150 hepatitis C virus (HCV)-positive case-patients, we identified 2 persons with HHpgV-1 viremia and a high frequency of human pegivirus (HPgV) viremia (14\%). Detection of HHpgV-1 and HPgV was concordant with parallel PCR-based screening using conserved primers matching groups 1 (HPgV) and 2 (HHPgV-1) nonstructural 3 region sequences. PCR identified 1 HHPgV-1-positive person with viremia from a group of 195 persons with hemophilia who had been exposed to nonvirally inactivated factor VII/IX; 18 (9\%) were HPgV-positive. Relative to HCV and HPgV, active infections with HHpgV-1 were infrequently detected in blood, even in groups that had substantial parenteral exposure. Our findings are consistent with lower transmissibility or higher rates of virus clearance for HHpgV-1 than for other bloodborne human flaviviruses.",

author = "David Bonsall and Gregory, \{William F\} and Ip, \{Camilla L C\} and Sharyne Donfield and James Iles and Ansari, \{M Azim\} and Paolo Piazza and Amy Trebes and Anthony Brown and John Frater and Pybus, \{Oliver G\} and Phillip Goulder and Paul Klenerman and Rory Bowden and Gomperts, \{Edward D\} and Eleanor Barnes and Amit Kapoor and Sharp, \{Colin P\} and Peter Simmonds",

year = "2016",

month = apr,

doi = "10.3201/eid2204.151812",

language = "English",

volume = "22",

pages = "671--678",

journal = "Emerging Infectious Diseases",

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Bonsall, D, Gregory, WF, Ip, CLC, Donfield, S, Iles, J, Ansari, MA, Piazza, P, Trebes, A, Brown, A, Frater, J, Pybus, OG, Goulder, P, Klenerman, P, Bowden, R, Gomperts, ED, Barnes, E, Kapoor, A, Sharp, CP & Simmonds, P 2016, 'Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR', Emerging Infectious Diseases, vol. 22, no. 4, pp. 671-678. https://doi.org/10.3201/eid2204.151812

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AU - Bonsall, David

AU - Gregory, William F

AU - Ip, Camilla L C

AU - Donfield, Sharyne

AU - Iles, James

AU - Ansari, M Azim

AU - Piazza, Paolo

AU - Trebes, Amy

AU - Brown, Anthony

AU - Frater, John

AU - Pybus, Oliver G

AU - Goulder, Phillip

AU - Klenerman, Paul

AU - Bowden, Rory

AU - Gomperts, Edward D

AU - Barnes, Eleanor

AU - Kapoor, Amit

AU - Sharp, Colin P

AU - Simmonds, Peter

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N2 - Next-generation sequencing has critical applications in virus discovery, diagnostics, and environmental surveillance. We used metagenomic sequence libraries for retrospective screening of plasma samples for the recently discovered human hepegivirus 1 (HHpgV-1). From a cohort of 150 hepatitis C virus (HCV)-positive case-patients, we identified 2 persons with HHpgV-1 viremia and a high frequency of human pegivirus (HPgV) viremia (14%). Detection of HHpgV-1 and HPgV was concordant with parallel PCR-based screening using conserved primers matching groups 1 (HPgV) and 2 (HHPgV-1) nonstructural 3 region sequences. PCR identified 1 HHPgV-1-positive person with viremia from a group of 195 persons with hemophilia who had been exposed to nonvirally inactivated factor VII/IX; 18 (9%) were HPgV-positive. Relative to HCV and HPgV, active infections with HHpgV-1 were infrequently detected in blood, even in groups that had substantial parenteral exposure. Our findings are consistent with lower transmissibility or higher rates of virus clearance for HHpgV-1 than for other bloodborne human flaviviruses.

AB - Next-generation sequencing has critical applications in virus discovery, diagnostics, and environmental surveillance. We used metagenomic sequence libraries for retrospective screening of plasma samples for the recently discovered human hepegivirus 1 (HHpgV-1). From a cohort of 150 hepatitis C virus (HCV)-positive case-patients, we identified 2 persons with HHpgV-1 viremia and a high frequency of human pegivirus (HPgV) viremia (14%). Detection of HHpgV-1 and HPgV was concordant with parallel PCR-based screening using conserved primers matching groups 1 (HPgV) and 2 (HHPgV-1) nonstructural 3 region sequences. PCR identified 1 HHPgV-1-positive person with viremia from a group of 195 persons with hemophilia who had been exposed to nonvirally inactivated factor VII/IX; 18 (9%) were HPgV-positive. Relative to HCV and HPgV, active infections with HHpgV-1 were infrequently detected in blood, even in groups that had substantial parenteral exposure. Our findings are consistent with lower transmissibility or higher rates of virus clearance for HHpgV-1 than for other bloodborne human flaviviruses.

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DO - 10.3201/eid2204.151812

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SN - 1080-6040

VL - 22

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EP - 678

JO - Emerging Infectious Diseases

JF - Emerging Infectious Diseases

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ER -

Evaluation of Viremia Frequencies of a Novel Human Pegivirus by Using Bioinformatic Screening and PCR

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ISP4 Pathogenesis and resistance in viral diseases of livestock

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