Evidence for the role of beta-1,3-glucanase in dicot seed germination.

R VOGELILANGE, C FRUNDT, C M HART, R BEFFA, F NAGY, F MEINS

Research output: Contribution to journalComment/debatepeer-review

Abstract

Class I beta-1,3-glucanases are antifungal vacuolar proteins implicated in plant defense that show developmental, hormonal, and pathogenesis-related regulation. The expression was studied in germinating tobacco seeds of a chimeric P-glucuronidase (GUS) reporter gene fused to 1.6 kb of the 5' flanking sequence of the tobacco class I beta-1,3-glucanase B (GLB) promoter. Histological staining for GUS activity showed that expression of the GLB promoter is highly localized in a specific zone of the endosperm in germinating seeds. The temporal and spatial patterns of GUS and beta-1,3-glucanase activity found, suggest a novel function for class I beta-1,3-glucanases during seed germination in a dicotyledonous plant.

Original languageEnglish
Pages (from-to)273-278
Number of pages6
JournalThe Plant Journal
Volume5
Issue number2
Publication statusPublished - Feb 1994

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