Evidence of tautomerism in 2-aminopurine from fluorescence lifetime measurements

R K Neely, S W Magennis, D T F Dryden, A C Jones

Research output: Contribution to journalArticlepeer-review


The fluorescence decay characteristics of 2-aminopurine (2AP) and 2-aminopurine riboside (2APr) have been investigated as a function of excitation and emission wavelength in aqueous and ethanolic solutions. Global analysis of the decay data shows that 2AP exists as two emitting species, whereas 2APr exists as a single species. This is attributed to 9H/7H tautomerism of 2AP. The proportion of 7H tautomer is estimated to be 20% in ethanol and 40% in water.

Original languageEnglish
Pages (from-to)17606-17610
Number of pages5
JournalJournal of Physical Chemistry B (Soft Condensed Matter and Biophysical Chemistry)
Issue number45
Publication statusPublished - 11 Nov 2004


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