Evolutionary history of the T cell receptor complex as revealed by small-spotted catshark (Scyliorhinus canicula)

Rita Pettinello, Anthony K Redmond, Christopher J Secombes, Daniel J Macqueen, Helen Dooley

Research output: Contribution to journalArticlepeer-review

Abstract

In every jawed vertebrate species studied so far, the T cell receptor (TCR) complex is composed of two different TCR chains (α/β or γ/δ) and a number of CD3 subunits responsible for transmitting signals into the T cell. In this study, we characterised all of the TCR and CD3 genes of small-spotted catshark (Scyliorhinus canicula) and analysed their expression in a broad range of tissues. While the TCR complex is highly conserved across jawed vertebrates, we identified a number of differences in catshark, most notably the presence of two copies of both TCRβ and CD3γδ, and the absence of a functionally-important proline rich region from CD3ε. We also demonstrate that TCRβ has duplicated independently multiple times in jawed vertebrate evolution, bringing additional diversity to the TCR complex. This study reveals new insights about the evolutionary history of the TCR complex and raises new avenues for future exploration.

Original languageEnglish
Pages (from-to)125-135
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume74
Early online date19 Apr 2017
DOIs
Publication statusE-pub ahead of print - 19 Apr 2017

Keywords

  • Animals
  • Biological Evolution
  • CD3 Complex/genetics
  • Evolution, Molecular
  • Fish Proteins/genetics
  • Gene Dosage
  • Gene Duplication
  • Immunity/genetics
  • Lymphocyte Activation
  • Multiprotein Complexes/metabolism
  • Receptors, Antigen, T-Cell/genetics
  • Sharks/immunology
  • Signal Transduction
  • T-Lymphocytes/physiology
  • Vertebrates

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