Ex vivo 18F-fluoride uptake and hydroxyapatite deposition in human coronary atherosclerosis

Alastair J  Moss; Alisia Sim; Philip D Adamson; Michael A.  Seidman; Jack Andrews; Mhairi Doris; Anoop Shah; Ralph Bouhaidar; Carlos José Alcaide-corral; Michelle C Williams; Jonathon A. Leipsic; Marc R Dweck; Vicky MacRae; David E Newby; Adriana A S Tavares; Stephanie L Sellers

doi:10.1038/s41598-020-77391-6

Ex vivo 18F-fluoride uptake and hydroxyapatite deposition in human coronary atherosclerosis

Alastair J Moss, Alisia Sim, Philip D Adamson, Michael A. Seidman, Jack Andrews, Mhairi Doris, Anoop Shah, Ralph Bouhaidar, Carlos José Alcaide-corral, Michelle C Williams, Jonathon A. Leipsic, Marc R Dweck, Vicky MacRae, David E Newby, Adriana A S Tavares, Stephanie L Sellers

Research output: Contribution to journal › Article › peer-review

Abstract

Early microcalcification is a feature of coronary plaques with an increased propensity to rupture and to cause acute coronary syndromes. In this ex vivo imaging study of coronary artery specimens, the non-invasive imaging radiotracer, 18F-fluoride, was highly selective for hydroxyapatite deposition in atherosclerotic coronary plaque. Specifically, coronary 18F-fluoride uptake had a high signal to noise ratio compared with surrounding myocardium that makes it feasible to identify coronary mineralisation activity. Areas of 18F-fluoride uptake are associated with osteopontin, an inflammation-associated glycophosphoprotein that mediates tissue mineralisation, and Runt-related transcription factor 2, a nuclear protein involved in osteoblastic differentiation. These results suggest that 18F-fluoride is a non-invasive imaging biomarker of active coronary atherosclerotic mineralisation.

Original language	English
Journal	Scientific Reports
Early online date	19 Nov 2020
DOIs	https://doi.org/10.1038/s41598-020-77391-6
Publication status	E-pub ahead of print - 19 Nov 2020

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s41598-020-77391-6Final published version, 1.62 MBLicence: Creative Commons: Attribution (CC-BY)

Edinburgh Preclinical Imaging
Moran, C. (Manager), Thomson, A. (Manager), Lennen, R. J. (Manager), Tavares, A. (Manager), Alcaide-Corral, C. J. (Manager), Morgan, T. (Other), Cranston, I. (Other) & O'Rourke, K. (Other)
Deanery of Clinical Sciences
Facility/equipment: Facility

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Moss, A. J., Sim, A., Adamson, P. D., Seidman, M. A., Andrews, J., Doris, M., Shah, A., Bouhaidar, R., Alcaide-corral, C. J., Williams, M. C., Leipsic, J. A., Dweck, M. R., MacRae, V., Newby, D. E., Tavares, A. A. S., & Sellers, S. L. (2020). Ex vivo 18F-fluoride uptake and hydroxyapatite deposition in human coronary atherosclerosis. Scientific Reports. Advance online publication. https://doi.org/10.1038/s41598-020-77391-6

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title = "Ex vivo 18F-fluoride uptake and hydroxyapatite deposition in human coronary atherosclerosis",

abstract = "Early microcalcification is a feature of coronary plaques with an increased propensity to rupture and to cause acute coronary syndromes. In this ex vivo imaging study of coronary artery specimens, the non-invasive imaging radiotracer, 18F-fluoride, was highly selective for hydroxyapatite deposition in atherosclerotic coronary plaque. Specifically, coronary 18F-fluoride uptake had a high signal to noise ratio compared with surrounding myocardium that makes it feasible to identify coronary mineralisation activity. Areas of 18F-fluoride uptake are associated with osteopontin, an inflammation-associated glycophosphoprotein that mediates tissue mineralisation, and Runt-related transcription factor 2, a nuclear protein involved in osteoblastic differentiation. These results suggest that 18F-fluoride is a non-invasive imaging biomarker of active coronary atherosclerotic mineralisation.",

author = "Moss, {Alastair J} and Alisia Sim and Adamson, {Philip D} and Seidman, {Michael A.} and Jack Andrews and Mhairi Doris and Anoop Shah and Ralph Bouhaidar and Alcaide-corral, {Carlos Jos{\'e}} and Williams, {Michelle C} and Leipsic, {Jonathon A.} and Dweck, {Marc R} and Vicky MacRae and Newby, {David E} and Tavares, {Adriana A S} and Sellers, {Stephanie L}",

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doi = "10.1038/s41598-020-77391-6",

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journal = "Scientific Reports",

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T1 - Ex vivo 18F-fluoride uptake and hydroxyapatite deposition in human coronary atherosclerosis

AU - Moss, Alastair J

AU - Sim, Alisia

AU - Adamson, Philip D

AU - Seidman, Michael A.

AU - Andrews, Jack

AU - Doris, Mhairi

AU - Shah, Anoop

AU - Bouhaidar, Ralph

AU - Alcaide-corral, Carlos José

AU - Williams, Michelle C

AU - Leipsic, Jonathon A.

AU - Dweck, Marc R

AU - MacRae, Vicky

AU - Newby, David E

AU - Tavares, Adriana A S

AU - Sellers, Stephanie L

PY - 2020/11/19

Y1 - 2020/11/19

N2 - Early microcalcification is a feature of coronary plaques with an increased propensity to rupture and to cause acute coronary syndromes. In this ex vivo imaging study of coronary artery specimens, the non-invasive imaging radiotracer, 18F-fluoride, was highly selective for hydroxyapatite deposition in atherosclerotic coronary plaque. Specifically, coronary 18F-fluoride uptake had a high signal to noise ratio compared with surrounding myocardium that makes it feasible to identify coronary mineralisation activity. Areas of 18F-fluoride uptake are associated with osteopontin, an inflammation-associated glycophosphoprotein that mediates tissue mineralisation, and Runt-related transcription factor 2, a nuclear protein involved in osteoblastic differentiation. These results suggest that 18F-fluoride is a non-invasive imaging biomarker of active coronary atherosclerotic mineralisation.

AB - Early microcalcification is a feature of coronary plaques with an increased propensity to rupture and to cause acute coronary syndromes. In this ex vivo imaging study of coronary artery specimens, the non-invasive imaging radiotracer, 18F-fluoride, was highly selective for hydroxyapatite deposition in atherosclerotic coronary plaque. Specifically, coronary 18F-fluoride uptake had a high signal to noise ratio compared with surrounding myocardium that makes it feasible to identify coronary mineralisation activity. Areas of 18F-fluoride uptake are associated with osteopontin, an inflammation-associated glycophosphoprotein that mediates tissue mineralisation, and Runt-related transcription factor 2, a nuclear protein involved in osteoblastic differentiation. These results suggest that 18F-fluoride is a non-invasive imaging biomarker of active coronary atherosclerotic mineralisation.

U2 - 10.1038/s41598-020-77391-6

DO - 10.1038/s41598-020-77391-6

M3 - Article

SN - 2045-2322

JO - Scientific Reports

JF - Scientific Reports

ER -

Ex vivo 18F-fluoride uptake and hydroxyapatite deposition in human coronary atherosclerosis

Abstract

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