Projects per year
Abstract / Description of output
The classical three-stage model of stochastic gene expression predicts the statistics of single cell mRNA and protein number fluctuations as a function of the rates of promoter switching, transcription, translation, degradation and dilution. While this model is easily simulated, its analytical solution remains an unsolved problem. Here we modify this model to explicitly include cell-cycle dynamics and then derive an exact solution for the time-dependent joint distribution of mRNA and protein numbers. We show large differences between this model and the classical model which captures cell-cycle effects implicitly via effective first-order dilution reactions. In particular we find that the Fano factor of protein numbers calculated from a population snapshot measurement are underestimated by the classical model whereas the correlation between mRNA and protein can be either over- or underestimated, depending on the timescales of mRNA degradation and promoter switching relative to the mean cell-cycle duration time.
Original language | English |
---|---|
Article number | 224102 |
Number of pages | 19 |
Journal | The Journal of Chemical Physics |
Volume | 159 |
Issue number | 22 |
Early online date | 8 Dec 2023 |
DOIs | |
Publication status | Published - 14 Dec 2023 |
Keywords / Materials (for Non-textual outputs)
- Proteins/metabolism
- RNA, Messenger/genetics
- Promoter Regions, Genetic/genetics
- Gene Expression
- Stochastic Processes
- Models, Genetic
Fingerprint
Dive into the research topics of 'Exact solution of a three-stage model of stochastic gene expression including cell-cycle dynamics'. Together they form a unique fingerprint.Projects
- 1 Finished