Expanding the genetic code of Drosophila melanogaster

Ambra Bianco; Fiona M. Townsley; Sebastian Greiss; Kathrin Lang; Jason W. Chin

doi:10.1038/NCHEMBIO.1043

Expanding the genetic code of Drosophila melanogaster

Ambra Bianco^*, Fiona M. Townsley, Sebastian Greiss, Kathrin Lang, Jason W. Chin

^*Corresponding author for this work

Deanery of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Genetic code expansion for unnatural amino acid mutagenesis has, until recently, been limited to cell culture. We demonstrate the site-specific incorporation of unnatural amino acids into proteins in Drosophila melanogaster at different developmental stages, in specific tissues and in a subset of cells within a tissue. This approach provides a foundation for probing and controlling processes in this established metazoan model organism with a new level of molecular precision.

Original language	English
Pages (from-to)	748-750
Number of pages	3
Journal	Nature Chemical Biology
Volume	8
Issue number	9
DOIs	https://doi.org/10.1038/NCHEMBIO.1043
Publication status	Published - Sept 2012

Keywords / Materials (for Non-textual outputs)

MAMMALIAN-CELLS
RECOMBINANT PROTEINS
AMINO-ACIDS
TRANSFORMATION
EXPRESSION

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10.1038/NCHEMBIO.1043

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title = "Expanding the genetic code of Drosophila melanogaster",

abstract = "Genetic code expansion for unnatural amino acid mutagenesis has, until recently, been limited to cell culture. We demonstrate the site-specific incorporation of unnatural amino acids into proteins in Drosophila melanogaster at different developmental stages, in specific tissues and in a subset of cells within a tissue. This approach provides a foundation for probing and controlling processes in this established metazoan model organism with a new level of molecular precision.",

keywords = "MAMMALIAN-CELLS, RECOMBINANT PROTEINS, AMINO-ACIDS, TRANSFORMATION, EXPRESSION",

author = "Ambra Bianco and Townsley, {Fiona M.} and Sebastian Greiss and Kathrin Lang and Chin, {Jason W.}",

year = "2012",

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T1 - Expanding the genetic code of Drosophila melanogaster

AU - Bianco, Ambra

AU - Townsley, Fiona M.

AU - Greiss, Sebastian

AU - Lang, Kathrin

AU - Chin, Jason W.

PY - 2012/9

Y1 - 2012/9

N2 - Genetic code expansion for unnatural amino acid mutagenesis has, until recently, been limited to cell culture. We demonstrate the site-specific incorporation of unnatural amino acids into proteins in Drosophila melanogaster at different developmental stages, in specific tissues and in a subset of cells within a tissue. This approach provides a foundation for probing and controlling processes in this established metazoan model organism with a new level of molecular precision.

AB - Genetic code expansion for unnatural amino acid mutagenesis has, until recently, been limited to cell culture. We demonstrate the site-specific incorporation of unnatural amino acids into proteins in Drosophila melanogaster at different developmental stages, in specific tissues and in a subset of cells within a tissue. This approach provides a foundation for probing and controlling processes in this established metazoan model organism with a new level of molecular precision.

KW - MAMMALIAN-CELLS

KW - RECOMBINANT PROTEINS

KW - AMINO-ACIDS

KW - TRANSFORMATION

KW - EXPRESSION

U2 - 10.1038/NCHEMBIO.1043

DO - 10.1038/NCHEMBIO.1043

M3 - Article

SN - 1552-4450

VL - 8

SP - 748

EP - 750

JO - Nature Chemical Biology

JF - Nature Chemical Biology

IS - 9

ER -

Expanding the genetic code of Drosophila melanogaster

Abstract

Keywords / Materials (for Non-textual outputs)

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