Expansion in scid mice of Epstein-Barr virus-associated post-transplantation lymphoproliferative disease biopsy material

Ingolfur Johannessen, Sunimali M Perera, Alice Gallagher, Paul A Hopwood, J Alero Thomas, Dorothy H Crawford

Research output: Contribution to journalArticlepeer-review

Abstract

Post-transplant lymphoproliferative disease (PTLD) biopsy material is rarely available in adequate quantity for research. Therefore, the present study was designed to expand biopsy material in scid mice. Epstein-Barr virus (EBV)+ve PTLD samples from five transplant patients were established in scid mice. PCR analysis of immunoglobulin gene rearrangements demonstrated that four of the five biopsies (80%) gave rise to scid tumours which represented the original tumour cell clones. Immunophenotyping showed that these four biopsies (and all scid tumours) expressed all EBV latent genes and a B lymphoblast phenotype; <or=26% T cells were found in the biopsy material whereas scid tumours showed a paucity of T lymphocytes. RT-PCR analysis revealed expression of IL-2, -4, -6, -10 and IFN-gamma in all tumour material, suggesting key roles for these factors in tumour growth. The results show that EBV+ve PTLD material can be expanded in scid mice giving rise to quantities of homogeneous malignant tissue sufficient for research studies.

Original languageEnglish
Pages (from-to)173-8
Number of pages6
JournalJournal of General Virology
Volume83
Issue numberPt 1
Publication statusPublished - Jan 2002

Keywords

  • Adolescent
  • Animals
  • Antigens, CD19
  • Antigens, Viral
  • Burkitt Lymphoma
  • Cell Division
  • Child
  • Child, Preschool
  • Cytokines
  • DNA-Binding Proteins
  • Epstein-Barr Virus Nuclear Antigens
  • Female
  • Gene Expression
  • Heart Transplantation
  • Herpesvirus 4, Human
  • Humans
  • Infant
  • Infectious Mononucleosis
  • Kidney Transplantation
  • Liver Transplantation
  • Male
  • Mice
  • Mice, SCID
  • Neoplasm Transplantation
  • Neoplasms, Experimental
  • Receptors, IgE
  • Trans-Activators
  • Transplantation, Heterologous
  • Tumor Cells, Cultured
  • Viral Matrix Proteins
  • Viral Proteins

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