Abstract / Description of output
Post-transplant lymphoproliferative disease (PTLD) biopsy material is rarely available in adequate quantity for research. Therefore, the present study was designed to expand biopsy material in scid mice. Epstein-Barr virus (EBV)+ve PTLD samples from five transplant patients were established in scid mice. PCR analysis of immunoglobulin gene rearrangements demonstrated that four of the five biopsies (80%) gave rise to scid tumours which represented the original tumour cell clones. Immunophenotyping showed that these four biopsies (and all scid tumours) expressed all EBV latent genes and a B lymphoblast phenotype; <or=26% T cells were found in the biopsy material whereas scid tumours showed a paucity of T lymphocytes. RT-PCR analysis revealed expression of IL-2, -4, -6, -10 and IFN-gamma in all tumour material, suggesting key roles for these factors in tumour growth. The results show that EBV+ve PTLD material can be expanded in scid mice giving rise to quantities of homogeneous malignant tissue sufficient for research studies.
| Original language | English |
|---|---|
| Pages (from-to) | 173-8 |
| Number of pages | 6 |
| Journal | Journal of General Virology |
| Volume | 83 |
| Issue number | Pt 1 |
| Publication status | Published - Jan 2002 |
Keywords / Materials (for Non-textual outputs)
- Adolescent
- Animals
- Antigens, CD19
- Antigens, Viral
- Burkitt Lymphoma
- Cell Division
- Child
- Child, Preschool
- Cytokines
- DNA-Binding Proteins
- Epstein-Barr Virus Nuclear Antigens
- Female
- Gene Expression
- Heart Transplantation
- Herpesvirus 4, Human
- Humans
- Infant
- Infectious Mononucleosis
- Kidney Transplantation
- Liver Transplantation
- Male
- Mice
- Mice, SCID
- Neoplasm Transplantation
- Neoplasms, Experimental
- Receptors, IgE
- Trans-Activators
- Transplantation, Heterologous
- Tumor Cells, Cultured
- Viral Matrix Proteins
- Viral Proteins