Experimental and Numerical Study of Electroporation Induced by Long Monopolar and Short Bipolar Pulses on Realistic 3D Irregularly Shaped Cells

Michele Alessandro Chiapperino; Luciano Mescia; Pietro Bia; Barbara Staresinic; Maja Cemazar; Vitalij Novickij; Aleksandr Tabasnikov; Stewart Smith; Janja  Dermol-Cerne; Damijan Miklavcic

doi:10.1109/TBME.2020.2971138

Experimental and Numerical Study of Electroporation Induced by Long Monopolar and Short Bipolar Pulses on Realistic 3D Irregularly Shaped Cells

Michele Alessandro Chiapperino, Luciano Mescia, Pietro Bia, Barbara Staresinic, Maja Cemazar, Vitalij Novickij, Aleksandr Tabasnikov, Stewart Smith, Janja Dermol-Cerne, Damijan Miklavcic

School of Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

In this paper, the effects of irreversible electroporation induced by rectangular long unipolar and short bipolar voltage pulses on 3D cells have been illustrated. The cells geometry was reconstructed starting from 3D images of real cells obtained using the confocal microscopy technique. A numerical model based on the Maxwell and the asymptotic Smoluchowski equations has been developed to calculate the induced transmembrane voltage and pore density on the plasma membrane of the real cells exposed to the pulsed electric field. Moreover, in the case of the high-frequency pulses, the dielectric dispersion of plasma membranes has been taken into account using the second-order Debye-based relationship. Several numerical simulations have been performed obtaining a good agreement between the numerical and experimental results.

Original language	English
Journal	IEEE Transactions on Biomedical Engineering
DOIs	https://doi.org/10.1109/TBME.2020.2971138
Publication status	Published - 3 Feb 2020

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10.1109/TBME.2020.2971138

SMART microsystems
Walton, A. (Principal Investigator)
EPSRC
1/04/10 → 28/02/15
Project: Research
RCUK Fellowship
Smith, S. (Principal Investigator), Anderton, S. (Co-investigator), Bagnaninchi, P. (Co-investigator), Blythe, R. (Co-investigator), Calvert, J. (Co-investigator), Downes, A. (Co-investigator), Frame, M. (Co-investigator), Hay, D. (Co-investigator), Popovic, N. (Co-investigator) & Rambaut, A. (Co-investigator)
EPSRC, STFC
1/10/06 → 30/06/13
Project: Research

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Chiapperino, M. A., Mescia, L., Bia, P., Staresinic, B., Cemazar, M., Novickij, V., Tabasnikov, A., Smith, S., Dermol-Cerne, J., & Miklavcic, D. (2020). Experimental and Numerical Study of Electroporation Induced by Long Monopolar and Short Bipolar Pulses on Realistic 3D Irregularly Shaped Cells. IEEE Transactions on Biomedical Engineering. https://doi.org/10.1109/TBME.2020.2971138

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title = "Experimental and Numerical Study of Electroporation Induced by Long Monopolar and Short Bipolar Pulses on Realistic 3D Irregularly Shaped Cells",

abstract = "In this paper, the effects of irreversible electroporation induced by rectangular long unipolar and short bipolar voltage pulses on 3D cells have been illustrated. The cells geometry was reconstructed starting from 3D images of real cells obtained using the confocal microscopy technique. A numerical model based on the Maxwell and the asymptotic Smoluchowski equations has been developed to calculate the induced transmembrane voltage and pore density on the plasma membrane of the real cells exposed to the pulsed electric field. Moreover, in the case of the high-frequency pulses, the dielectric dispersion of plasma membranes has been taken into account using the second-order Debye-based relationship. Several numerical simulations have been performed obtaining a good agreement between the numerical and experimental results.",

author = "Chiapperino, {Michele Alessandro} and Luciano Mescia and Pietro Bia and Barbara Staresinic and Maja Cemazar and Vitalij Novickij and Aleksandr Tabasnikov and Stewart Smith and Janja Dermol-Cerne and Damijan Miklavcic",

year = "2020",

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doi = "10.1109/TBME.2020.2971138",

language = "English",

journal = "IEEE Transactions on Biomedical Engineering",

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Chiapperino, MA, Mescia, L, Bia, P, Staresinic, B, Cemazar, M, Novickij, V, Tabasnikov, A, Smith, S, Dermol-Cerne, J & Miklavcic, D 2020, 'Experimental and Numerical Study of Electroporation Induced by Long Monopolar and Short Bipolar Pulses on Realistic 3D Irregularly Shaped Cells', IEEE Transactions on Biomedical Engineering. https://doi.org/10.1109/TBME.2020.2971138

TY - JOUR

T1 - Experimental and Numerical Study of Electroporation Induced by Long Monopolar and Short Bipolar Pulses on Realistic 3D Irregularly Shaped Cells

AU - Chiapperino, Michele Alessandro

AU - Mescia, Luciano

AU - Bia, Pietro

AU - Staresinic, Barbara

AU - Cemazar, Maja

AU - Novickij, Vitalij

AU - Tabasnikov, Aleksandr

AU - Smith, Stewart

AU - Dermol-Cerne, Janja

AU - Miklavcic, Damijan

PY - 2020/2/3

Y1 - 2020/2/3

N2 - In this paper, the effects of irreversible electroporation induced by rectangular long unipolar and short bipolar voltage pulses on 3D cells have been illustrated. The cells geometry was reconstructed starting from 3D images of real cells obtained using the confocal microscopy technique. A numerical model based on the Maxwell and the asymptotic Smoluchowski equations has been developed to calculate the induced transmembrane voltage and pore density on the plasma membrane of the real cells exposed to the pulsed electric field. Moreover, in the case of the high-frequency pulses, the dielectric dispersion of plasma membranes has been taken into account using the second-order Debye-based relationship. Several numerical simulations have been performed obtaining a good agreement between the numerical and experimental results.

AB - In this paper, the effects of irreversible electroporation induced by rectangular long unipolar and short bipolar voltage pulses on 3D cells have been illustrated. The cells geometry was reconstructed starting from 3D images of real cells obtained using the confocal microscopy technique. A numerical model based on the Maxwell and the asymptotic Smoluchowski equations has been developed to calculate the induced transmembrane voltage and pore density on the plasma membrane of the real cells exposed to the pulsed electric field. Moreover, in the case of the high-frequency pulses, the dielectric dispersion of plasma membranes has been taken into account using the second-order Debye-based relationship. Several numerical simulations have been performed obtaining a good agreement between the numerical and experimental results.

U2 - 10.1109/TBME.2020.2971138

DO - 10.1109/TBME.2020.2971138

M3 - Article

SN - 0018-9294

JO - IEEE Transactions on Biomedical Engineering

JF - IEEE Transactions on Biomedical Engineering

ER -

Experimental and Numerical Study of Electroporation Induced by Long Monopolar and Short Bipolar Pulses on Realistic 3D Irregularly Shaped Cells

Abstract

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RCUK Fellowship

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