Experimental design for single-cell RNA sequencing

Jeanette Baran-Gale; Tamir Chandra; Kristina Kirschner

doi:10.1093/bfgp/elx035

Experimental design for single-cell RNA sequencing

Jeanette Baran-Gale, Tamir Chandra, Kristina Kirschner

Research output: Contribution to journal › Review article › peer-review

Abstract / Description of output

Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.

Original language	English
Journal	Briefings in functional genomics
DOIs	https://doi.org/10.1093/bfgp/elx035
Publication status	Published - 8 Nov 2017

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Journal Article

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10.1093/bfgp/elx035

Baran-Gale J Experimental design...
V C The Author 2017. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/ 4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Final published version, 481 KBLicence: Creative Commons: Attribution (CC-BY)
Experimental design for single cell RNA sequencing

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title = "Experimental design for single-cell RNA sequencing",

abstract = "Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.",

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AU - Baran-Gale, Jeanette

AU - Chandra, Tamir

AU - Kirschner, Kristina

PY - 2017/11/8

Y1 - 2017/11/8

N2 - Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.

AB - Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.

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DO - 10.1093/bfgp/elx035

M3 - Review article

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SN - 2041-2657

JO - Briefings in functional genomics

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Experimental design for single-cell RNA sequencing

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