Experimental design for single-cell RNA sequencing

Jeanette Baran-Gale, Tamir Chandra, Kristina Kirschner

Research output: Contribution to journalReview articlepeer-review

Abstract

Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.

Original languageEnglish
JournalBriefings in functional genomics
DOIs
Publication statusPublished - 8 Nov 2017

Keywords

  • Journal Article

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