Exposure to chemical cocktails before or after conception - The effect of timing on ovarian development

Michelle Bellingham, Maria R Amezaga, Beatrice Mandon-Pepin, Christopher J B Speers, Carol E Kyle, Neil P Evans, Richard M Sharpe, Corinne Cotinot, Stewart M Rhind, Paul A Fowler

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Exposure of female fetuses to environmental chemicals (ECs) during pregnancy results in a disturbed ovarian adult phenotype. We investigated the influence of pre- and/or post-conception exposure to low-level mixtures of ECs on the structure and function of the fetal ovine ovary. We examined ovarian morphology, expression of oocyte and granulosa cell-specific genes and proteome. Female fetuses were collected at day 110 of gestation, from dams exposed continuously until, and after mating, by grazing in pastures treated with sewage sludge as a fertiliser (TT) or in control fields treated with inorganic fertiliser (CC). In addition, in a cross-over design, fetal ovaries were collected from dams maintained on sludge pastures up to the time of mating but then transferred to control pastures (TC) and, reciprocally, those transferred from control to treated pastures at mating (CT). On examination, the proportion of type 1a follicles (activating primordial follicles) was significantly lower in animals from the CT groups compared with CC and TT groups (P
Original languageEnglish
Pages (from-to)156-172
Number of pages17
JournalMolecular and Cellular Endocrinology
Issue number1-2
Early online date20 Jun 2013
Publication statusPublished - 25 Aug 2013


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