Expressed gene sequences of two variants of sheep interleukin-25

Anton Gossner, Anna Peers, Virginia Venturina, John Hopkins

Research output: Contribution to journalArticlepeer-review

Abstract

This report describes the cloning and characterization of sheep interleukin-25 (IL25) expressed gene sequences and shows that, like humans, sheep express two transcript variants of IL25. Transcript variant 1 (IL25v1) has a 510 bp open reading frame encoding a 169 amino acid polypeptide with a calculated M-r 19,200. The 498 bp IL25v2 encodes a 165 amino acid polypeptide with a calculated M-r 18,710; both with an isoelectric point equal to 8.0307. The additional 12 bp of IL-25 isoform 1 are at the 5' end and encode an MYQA peptide, otherwise their sequences are identical. Phylogenetic analysis shows that both sheep IL-25 isoforms are most closely related to cattle and pig IL-25. (C) 2010 Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)319-323
Number of pages5
JournalVeterinary Immunology and Immunopathology
Volume139
Issue number2-4
DOIs
Publication statusPublished - 15 Feb 2011

Keywords

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Gene Expression Regulation/physiology
  • Genetic Variation
  • Interleukin-17/genetics
  • Interleukin-17/metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Protein Isoforms
  • Sheep/metabolism

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