Expressed gene sequences of two variants of sheep interleukin-25

Anton Gossner; Anna Peers; Virginia Venturina; John Hopkins

doi:10.1016/j.vetimm.2010.10.019

Expressed gene sequences of two variants of sheep interleukin-25

Anton Gossner, Anna Peers, Virginia Venturina, John Hopkins

Research output: Contribution to journal › Article › peer-review

Abstract

This report describes the cloning and characterization of sheep interleukin-25 (IL25) expressed gene sequences and shows that, like humans, sheep express two transcript variants of IL25. Transcript variant 1 (IL25v1) has a 510 bp open reading frame encoding a 169 amino acid polypeptide with a calculated M-r 19,200. The 498 bp IL25v2 encodes a 165 amino acid polypeptide with a calculated M-r 18,710; both with an isoelectric point equal to 8.0307. The additional 12 bp of IL-25 isoform 1 are at the 5' end and encode an MYQA peptide, otherwise their sequences are identical. Phylogenetic analysis shows that both sheep IL-25 isoforms are most closely related to cattle and pig IL-25. (C) 2010 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	319-323
Number of pages	5
Journal	Veterinary Immunology and Immunopathology
Volume	139
Issue number	2-4
DOIs	https://doi.org/10.1016/j.vetimm.2010.10.019
Publication status	Published - 15 Feb 2011

Keywords

Amino Acid Sequence
Animals
Base Sequence
Gene Expression Regulation/physiology
Genetic Variation
Interleukin-17/genetics
Interleukin-17/metabolism
Molecular Sequence Data
Phylogeny
Protein Isoforms
Sheep/metabolism

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10.1016/j.vetimm.2010.10.019

Elsevier Editorial SystemSubmitted manuscript, 985 KB

http://www.sciencedirect.com/science/article/pii/S0165242710003557

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title = "Expressed gene sequences of two variants of sheep interleukin-25",

abstract = "This report describes the cloning and characterization of sheep interleukin-25 (IL25) expressed gene sequences and shows that, like humans, sheep express two transcript variants of IL25. Transcript variant 1 (IL25v1) has a 510 bp open reading frame encoding a 169 amino acid polypeptide with a calculated M-r 19,200. The 498 bp IL25v2 encodes a 165 amino acid polypeptide with a calculated M-r 18,710; both with an isoelectric point equal to 8.0307. The additional 12 bp of IL-25 isoform 1 are at the 5' end and encode an MYQA peptide, otherwise their sequences are identical. Phylogenetic analysis shows that both sheep IL-25 isoforms are most closely related to cattle and pig IL-25. (C) 2010 Elsevier B.V. All rights reserved.",

keywords = "Amino Acid Sequence, Animals, Base Sequence, Gene Expression Regulation/physiology, Genetic Variation, Interleukin-17/genetics, Interleukin-17/metabolism, Molecular Sequence Data, Phylogeny, Protein Isoforms, Sheep/metabolism",

author = "Anton Gossner and Anna Peers and Virginia Venturina and John Hopkins",

year = "2011",

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doi = "10.1016/j.vetimm.2010.10.019",

language = "English",

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journal = "Veterinary Immunology and Immunopathology",

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T1 - Expressed gene sequences of two variants of sheep interleukin-25

AU - Gossner, Anton

AU - Peers, Anna

AU - Venturina, Virginia

AU - Hopkins, John

PY - 2011/2/15

Y1 - 2011/2/15

N2 - This report describes the cloning and characterization of sheep interleukin-25 (IL25) expressed gene sequences and shows that, like humans, sheep express two transcript variants of IL25. Transcript variant 1 (IL25v1) has a 510 bp open reading frame encoding a 169 amino acid polypeptide with a calculated M-r 19,200. The 498 bp IL25v2 encodes a 165 amino acid polypeptide with a calculated M-r 18,710; both with an isoelectric point equal to 8.0307. The additional 12 bp of IL-25 isoform 1 are at the 5' end and encode an MYQA peptide, otherwise their sequences are identical. Phylogenetic analysis shows that both sheep IL-25 isoforms are most closely related to cattle and pig IL-25. (C) 2010 Elsevier B.V. All rights reserved.

AB - This report describes the cloning and characterization of sheep interleukin-25 (IL25) expressed gene sequences and shows that, like humans, sheep express two transcript variants of IL25. Transcript variant 1 (IL25v1) has a 510 bp open reading frame encoding a 169 amino acid polypeptide with a calculated M-r 19,200. The 498 bp IL25v2 encodes a 165 amino acid polypeptide with a calculated M-r 18,710; both with an isoelectric point equal to 8.0307. The additional 12 bp of IL-25 isoform 1 are at the 5' end and encode an MYQA peptide, otherwise their sequences are identical. Phylogenetic analysis shows that both sheep IL-25 isoforms are most closely related to cattle and pig IL-25. (C) 2010 Elsevier B.V. All rights reserved.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Gene Expression Regulation/physiology

KW - Genetic Variation

KW - Interleukin-17/genetics

KW - Interleukin-17/metabolism

KW - Molecular Sequence Data

KW - Phylogeny

KW - Protein Isoforms

KW - Sheep/metabolism

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U2 - 10.1016/j.vetimm.2010.10.019

DO - 10.1016/j.vetimm.2010.10.019

M3 - Article

VL - 139

SP - 319

EP - 323

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

SN - 0165-2427

IS - 2-4

ER -

Expressed gene sequences of two variants of sheep interleukin-25

Abstract

Keywords

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Ark Genomics

Molecular pathogenesis of sheep scrapie: A functional genomic approach to understanding the molecular pathogenesis of sheep scrapie

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Expressed gene sequences of two variants of sheep interleukin-25

Abstract

Keywords

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Ark Genomics

Molecular pathogenesis of sheep scrapie: A functional genomic approach to understanding the molecular pathogenesis of sheep scrapie

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