Expression analysis of proline rich 15 (Prr15) in mouse and human gastrointestinal tumors

Dominique Meunier, Kalicharan Patra, Ron Smits, Andrea Hägebarth, Angela Lüttges, Rolf Jaussi, Matthew J Wieduwilt, Leticia Quintanilla-Fend, Heinz Himmelbauer, Riccardo Fodde, Reinald H Fundele

Research output: Contribution to journalArticlepeer-review

Abstract

Proline rich 15 (Prr15), which encodes a protein of unknown function, is expressed almost exclusively in postmitotic cells both during fetal development and in adult tissues, such as the intestinal epithelium and the testis. To determine if this specific expression is lost in intestinal neoplasias, we examined Prr15 expression by in situ hybridization (ISH) on mouse intestinal tumors caused by different gene mutations, and on human colorectal cancer (CRC) samples. Prr15/PRR15 expression was consistently observed in mouse gastrointestinal (GI) tumors caused by mutations in the Apc gene, as well as in several advanced stage human CRCs. In contrast, no Prr15 expression was detected in intestinal tumors derived from mice carrying mutations in the Smad3, Smad4, or Cdkn1b genes. These findings, combined with the fact that a majority of sporadic human CRCs carry APC mutations, strongly suggest that the expression of Prr15/PRR15 in mouse and human GI tumors is linked, directly or indirectly, to the absence of the APC protein or, more generally, to the disruption of the Wnt signaling pathway.

Original languageEnglish
Pages (from-to)8-15
Number of pages8
JournalMolecular carcinogenesis
Volume50
Issue number1
DOIs
Publication statusPublished - Jan 2011

Keywords

  • Adenocarcinoma/metabolism
  • Adenocarcinoma, Mucinous/metabolism
  • Adenomatous Polyposis Coli Protein/metabolism
  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Blotting, Northern
  • Cyclin-Dependent Kinase Inhibitor p27/physiology
  • Female
  • Gastrointestinal Neoplasms/metabolism
  • Humans
  • Immunoenzyme Techniques
  • In Situ Hybridization
  • Lymphatic Metastasis
  • Male
  • Mice
  • Mice, Inbred C3H
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Middle Aged
  • Mutation/genetics
  • Nuclear Proteins/metabolism
  • Nucleic Acid Hybridization
  • Proline/genetics
  • Proteins/genetics
  • RNA, Neoplasm/genetics
  • Signal Transduction
  • Smad3 Protein/physiology
  • Smad4 Protein/physiology

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