Expression and electrophysiological identification of the receptor for bombesin and gastrin-releasing peptide in Xenopus laevis oocytes injected with polyA+ RNA from rat brain

W Meyerhof, S D Morley, D Richter

Research output: Contribution to journalArticlepeer-review

Abstract

The receptor for bombesin and the related peptide, gastrin-releasing peptide (GRP) has been induced in frog oocytes by injection of polyA+ RNA from rat brain. The primed oocytes responded to peptides of the bombesin family (GRP, neuromedin C of bombesin) by showing dose-dependent oscillations in membrane currents as recorded by the voltage-clamp method. The induced membrane changes were suppressed when oocytes were pretreated with a bombesin-receptor antagonist.

Original languageEnglish
Pages (from-to)109-12
Number of pages4
JournalFEBS Letters
Volume239
Issue number1
Publication statusPublished - 24 Oct 1988

Keywords

  • Animals
  • Bombesin
  • Brain
  • Cell Membrane
  • Female
  • Gastrin-Releasing Peptide
  • Membrane Potentials
  • Microinjections
  • Oocytes
  • Peptides
  • Poly A
  • Protein Biosynthesis
  • RNA
  • RNA, Messenger
  • Rats
  • Receptors, Bombesin
  • Receptors, Neurotransmitter
  • Xenopus laevis

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