Expression and functional characterization of bovine receptor activator of NF-κB ligand (RANKL)

Ambre F Chapuis, Omar A Alfituri, Jayne C Hope, Jo Stevens, Jo Moore, Kevin Mclean, Dorota Androscuk, Inga Dry

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

Receptor activator of nuclear factor Kappa-B Ligand (RANKL) is a member of the tumor necrosis factor ligand (TNF) family involved in immune responses and immunomodulation. Expressed in various cells types around the body, RANKL plays a crucial role in bone remodeling and development of the thymus, lymph nodes and mammary glands. Research in other species demonstrates that RANKL is required for the development of microfold cells (M cells) in the gut, however limited information specific to cattle is available. Cloning and expression of bovine RANKL (BoRANKL) was carried out and bioactivity of the protein was demonstrated in the induction of osteoclast differentiation from both bovine and ovine bone marrow cells. The effects of BoRANKL on particle uptake in bovine enteroids was also assessed. The production of cross-reactive bovine RANKL protein will enable further investigations into cell differentiation using the available ruminant organoid systems, and their role in investigating host-pathogen interactions in cattle and sheep.

Original languageEnglish
Article number110705
Pages (from-to)1-8
Number of pages8
JournalVeterinary Immunology and Immunopathology
Early online date21 Dec 2023
Publication statusE-pub ahead of print - 21 Dec 2023


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