Expression and genomic characterization of protein phosphatase inhibitor-1: a novel marker for mesothelium in the mouse

L McLaren, S Boyle, J O Mason, J B Bard

Research output: Contribution to journalArticlepeer-review

Abstract

Protein phosphatase inhibitor-1 (inhibitor-1 or I-1) is involved in signal transduction and is an endogenous inhibitor of protein phosphatase-1. The mouse I-1 protein sequence has been deduced from cDNA and is strongly homologous to the published rat sequence. A mouse genomic library was screened, and the I-1 gene was characterized and localized by fluorescent in situ hybridization (FISH) to chromosome 15F. Protein expression in a range of embryonic and adult tissue was analysed using confocal microscopy. Inhibitor-1 is expressed by: the coelomic epithelium; the epithelial bounding layer of cells of the kidney, lung, liver, heart, intestine and gonad; and the surface ectoderm. The blast cells of the kidney do not express I-1. We conclude that I-1 is a marker for mesothelium.
Original languageEnglish
Pages (from-to)237-41
Number of pages5
JournalMechanisms of Development
Volume96
Issue number2
Publication statusPublished - 2000

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