Expression level of heterologous proteins in Pichia pastoris is influenced by flask design

F Villatte, A S Hussein, T T Bachmann, R D Schmid, Till Bachmann

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

The yeast Pichia pastoris is a convenient production system that enables expression of heterologous proteins in high amounts. As a fermentation method, shaking flasks are very popular because of their simplicity of handling and their low cost. We compared the expression level of the enzyme acetylcholinesterase in a transformed strain of P. pastoris grown in different flasks, presenting various designs but all with the same volume. A several-thousand-fold difference appeared in the expression levels; and the results could not be explained by differences between the flasks in the oxygenation of the medium. The data show that flask design is an important factor to consider for optimising fermentation processes.
Original languageEnglish
Pages (from-to)463-5
Number of pages3
JournalApplied Microbiology and Biotechnology
Volume55
Issue number4
Publication statusPublished - 2001

Keywords / Materials (for Non-textual outputs)

  • Acetylcholinesterase
  • Bioreactors
  • Equipment Design
  • Fermentation
  • Gene Expression
  • Oxygen
  • Pichia
  • Recombinant Proteins

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