Expression of c-myc is down-regulated as mouse mammary epithelial cells become confluent

S Grolli, P Accornero, R Ramoni, G Donofrio, C B Whitelaw

Research output: Contribution to journalArticlepeer-review

Abstract / Description of output

We have investigated the expression profile of c-myc in the mammary gland. During pregnancy when the gland is actively growing c-myc mRNA was present, while in the differentiated lactating gland no c-myc mRNA was detected. This correspondence between the differentiation state and c-myc mRNA levels in the mouse mammary gland in vivo was paralleled by HC11 mouse mammary epithelial cells in vitro. Firstly, the endogenous c-myc gene was suppressed in confluent compared to growing HC11 cells. In addition, treating the cells with lactogenic hormones did not induce c-myc expression. Secondly, a stably transfected c-myc-CAT reporter construct was similarly down-regulated. Furthermore, using this transfection model, we demonstrate that the mechanism(s) involved in regulating c-myc expression must act through the P1 and P2 core promoter and exon 1. Finally, we demonstrate that suppression of c-myc expression occurs when HC11 cells growth-arrest as they become confluent.
Original languageEnglish
Pages (from-to)566-9
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume239
Issue number2
DOIs
Publication statusPublished - 20 Oct 1997

Keywords / Materials (for Non-textual outputs)

  • Animals
  • Cell Differentiation
  • Cell Division
  • Down-Regulation
  • Epithelial Cells
  • Exons
  • Gene Expression Regulation
  • Genes, myc
  • Mammary Glands, Animal
  • Mice
  • Milk Proteins
  • Promoter Regions, Genetic
  • Transfection

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