Expression of Intermediate Filaments in the Developing Testis and Testicular Germ Cell Cancer

Maria E. Camacho-Moll; Leendert H. J. Looijenga; Roland Donat; Chitranjan J. Shukla; Anne Jørgensen; Rod T. Mitchell

doi:10.3390/cancers14225479

Expression of Intermediate Filaments in the Developing Testis and Testicular Germ Cell Cancer

Maria E. Camacho-Moll, Leendert H. J. Looijenga, Roland Donat, Chitranjan J. Shukla, Anne Jørgensen, Rod T. Mitchell

Research output: Contribution to journal › Article › peer-review

Abstract

Cytokeratin and desmin expression have been associated with Sertoli cell maturity and the development of testicular germ cell cancer (TGCC). Thus, the present study aimed to characterize the expression of these intermediate filaments in normal testis development and TGCC. Cytokeratin and desmin were determined by immunohistochemistry and immunofluorescence in human fetal, and adult testis and tissue from patients with pre-invasive germ cell neoplasia in-situ (GCNIS) or invasive TGCC. Desmin was expressed in Sertoli cells of the human fetal testis, and the proportion of desmin expressing Sertoli cells was significantly reduced in the second trimester, compared with the first trimester (31.14% vs. 6.74%, p = 0.0016). Additionally, Desmin was expressed in the majority of Sertoli cells in the adult testis and TGCC samples. Cytokeratin was detected in Sertoli cells of human fetal testis but was not expressed in Sertoli cells of human adult testis. In patients with TGCC, cytokeratin was not expressed in Sertoli cells in tubules with active spermatogenesis but was detected in Sertoli cells in tubules containing GCNIS cells in patients with both pre-invasive and invasive TGCC. In conclusion, desmin was not associated with Sertoli cell maturation or progression to TGCC. However, cytokeratin appeared to be an indicator of impaired Sertoli cell maturation. View Full-Text

Original language	English
Article number	5479
Journal	Cancers
Volume	14
Issue number	22
DOIs	https://doi.org/10.3390/cancers14225479
Publication status	Published - 8 Nov 2022

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https://www.mdpi.com/2072-6694/14/22/5479

1 Active
2 Finished

Protecting spermatogonial stem cells from chemotherapy-induced damage for fertility preservation in childhood cancer
Carragher, N.
MRC
1/05/19 → 30/04/23
Project: Research
MRC Centre for Reproductive Health at the University of Edinburgh
Pollard, J.
MRC
12/09/16 → 11/09/22
Project: Research
Intermediate Clinical Fellowship - The germ stem cell niche in the human testis and the origins of Testicular Cancer
Mitchell, R.
UK-based charities
1/10/12 → 31/03/17
Project: Research

Cite this

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title = "Expression of Intermediate Filaments in the Developing Testis and Testicular Germ Cell Cancer",

abstract = " Cytokeratin and desmin expression have been associated with Sertoli cell maturity and the development of testicular germ cell cancer (TGCC). Thus, the present study aimed to characterize the expression of these intermediate filaments in normal testis development and TGCC. Cytokeratin and desmin were determined by immunohistochemistry and immunofluorescence in human fetal, and adult testis and tissue from patients with pre-invasive germ cell neoplasia in-situ (GCNIS) or invasive TGCC. Desmin was expressed in Sertoli cells of the human fetal testis, and the proportion of desmin expressing Sertoli cells was significantly reduced in the second trimester, compared with the first trimester (31.14% vs. 6.74%, p = 0.0016). Additionally, Desmin was expressed in the majority of Sertoli cells in the adult testis and TGCC samples. Cytokeratin was detected in Sertoli cells of human fetal testis but was not expressed in Sertoli cells of human adult testis. In patients with TGCC, cytokeratin was not expressed in Sertoli cells in tubules with active spermatogenesis but was detected in Sertoli cells in tubules containing GCNIS cells in patients with both pre-invasive and invasive TGCC. In conclusion, desmin was not associated with Sertoli cell maturation or progression to TGCC. However, cytokeratin appeared to be an indicator of impaired Sertoli cell maturation. View Full-Text ",

author = "Camacho-Moll, {Maria E.} and Looijenga, {Leendert H. J.} and Roland Donat and Shukla, {Chitranjan J.} and Anne J{\o}rgensen and Mitchell, {Rod T.}",

note = "Funding Information: M.E.C.M. was funded by a PhD studentship award (CONACYT, Mexico, reference number: 214983) and a University of Edinburgh Charles Darwin Scholarship (reference number s1100244). This project was funded by a Wellcome Trust Intermediate Clinical Fellowship (Grant no. 098522) awarded to R.T.M. R.T.M. was supported by a UK Research and Innovation (UKRI) Future Leaders Fellowship (MR/S017151/1). This work was undertaken in the MRC Centre for Reproductive Health funded by the Grant MR/N022556/1. Publisher Copyright: {\textcopyright} 2022 by the authors.",

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month = nov,

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doi = "10.3390/cancers14225479",

language = "English",

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T1 - Expression of Intermediate Filaments in the Developing Testis and Testicular Germ Cell Cancer

AU - Camacho-Moll, Maria E.

AU - Looijenga, Leendert H. J.

AU - Donat, Roland

AU - Shukla, Chitranjan J.

AU - Jørgensen, Anne

AU - Mitchell, Rod T.

N1 - Funding Information: M.E.C.M. was funded by a PhD studentship award (CONACYT, Mexico, reference number: 214983) and a University of Edinburgh Charles Darwin Scholarship (reference number s1100244). This project was funded by a Wellcome Trust Intermediate Clinical Fellowship (Grant no. 098522) awarded to R.T.M. R.T.M. was supported by a UK Research and Innovation (UKRI) Future Leaders Fellowship (MR/S017151/1). This work was undertaken in the MRC Centre for Reproductive Health funded by the Grant MR/N022556/1. Publisher Copyright: © 2022 by the authors.

PY - 2022/11/8

Y1 - 2022/11/8

N2 - Cytokeratin and desmin expression have been associated with Sertoli cell maturity and the development of testicular germ cell cancer (TGCC). Thus, the present study aimed to characterize the expression of these intermediate filaments in normal testis development and TGCC. Cytokeratin and desmin were determined by immunohistochemistry and immunofluorescence in human fetal, and adult testis and tissue from patients with pre-invasive germ cell neoplasia in-situ (GCNIS) or invasive TGCC. Desmin was expressed in Sertoli cells of the human fetal testis, and the proportion of desmin expressing Sertoli cells was significantly reduced in the second trimester, compared with the first trimester (31.14% vs. 6.74%, p = 0.0016). Additionally, Desmin was expressed in the majority of Sertoli cells in the adult testis and TGCC samples. Cytokeratin was detected in Sertoli cells of human fetal testis but was not expressed in Sertoli cells of human adult testis. In patients with TGCC, cytokeratin was not expressed in Sertoli cells in tubules with active spermatogenesis but was detected in Sertoli cells in tubules containing GCNIS cells in patients with both pre-invasive and invasive TGCC. In conclusion, desmin was not associated with Sertoli cell maturation or progression to TGCC. However, cytokeratin appeared to be an indicator of impaired Sertoli cell maturation. View Full-Text

AB - Cytokeratin and desmin expression have been associated with Sertoli cell maturity and the development of testicular germ cell cancer (TGCC). Thus, the present study aimed to characterize the expression of these intermediate filaments in normal testis development and TGCC. Cytokeratin and desmin were determined by immunohistochemistry and immunofluorescence in human fetal, and adult testis and tissue from patients with pre-invasive germ cell neoplasia in-situ (GCNIS) or invasive TGCC. Desmin was expressed in Sertoli cells of the human fetal testis, and the proportion of desmin expressing Sertoli cells was significantly reduced in the second trimester, compared with the first trimester (31.14% vs. 6.74%, p = 0.0016). Additionally, Desmin was expressed in the majority of Sertoli cells in the adult testis and TGCC samples. Cytokeratin was detected in Sertoli cells of human fetal testis but was not expressed in Sertoli cells of human adult testis. In patients with TGCC, cytokeratin was not expressed in Sertoli cells in tubules with active spermatogenesis but was detected in Sertoli cells in tubules containing GCNIS cells in patients with both pre-invasive and invasive TGCC. In conclusion, desmin was not associated with Sertoli cell maturation or progression to TGCC. However, cytokeratin appeared to be an indicator of impaired Sertoli cell maturation. View Full-Text

U2 - 10.3390/cancers14225479

DO - 10.3390/cancers14225479

M3 - Article

VL - 14

JO - Cancers

JF - Cancers

SN - 2072-6694

IS - 22

M1 - 5479

ER -

Expression of Intermediate Filaments in the Developing Testis and Testicular Germ Cell Cancer

Abstract

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Projects

Protecting spermatogonial stem cells from chemotherapy-induced damage for fertility preservation in childhood cancer

MRC Centre for Reproductive Health at the University of Edinburgh

Intermediate Clinical Fellowship - The germ stem cell niche in the human testis and the origins of Testicular Cancer

Cite this