Expression of membrane-associated proteins within single emulsion cell facsimiles

Mayuree Chanasakulniyom, Chiara Martino, David Paterson, Louise Horsfall, Susan Rosser, Jonathan M. Cooper

Research output: Contribution to journalArticlepeer-review

Abstract

MreB is a structural membrane-associated protein which is one of the key components of the bacterial cytoskeleton. Although it plays an important role in shape maintenance of rod-like bacteria, the understanding of its mechanism of action is still not fully understood. This study shows how segmented flow and microdroplet technology can be used as a new tool for biological in vitro investigation of this protein. In this paper, we demonstrate cell-free expression in a single emulsion system to express red fluorescence protein (RFP) and MreB linked RFP (MreB-RFP). We follow the aggregation and localisation of the fusion protein MreB-RFP in this artificial cell-like environment. The expression of MreB-RFP in single emulsion droplets leads to the formation of micrometer-scale protein patches distributed at the water/oil interface.

Original languageEnglish
Pages (from-to)2939-2943
Number of pages5
JournalAnalyst
Volume137
Issue number13
DOIs
Publication statusPublished - 2012

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